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Titolo:
IDENTIFICATION OF 2 TYPES OF BETA-COP VESICLES IN THE GOLGI-COMPLEX OF RAT SPERMATIDS
Autore:
MARTINEZMENARGUEZ JA; GEUZE HJ; BALLESTA J;
Indirizzi:
UNIV MURCIA,SCH MED,DEPT CELL BIOL,SECT HISTOL & GEN EMBRYOL E-30071 MURCIA SPAIN UNIV UTRECHT,CTR ELECTRON MICROSCOPY,DEPT CELL BIOL UTRECHT NETHERLANDS
Titolo Testata:
European journal of cell biology
fascicolo: 2, volume: 71, anno: 1996,
pagine: 137 - 143
SICI:
0171-9335(1996)71:2<137:IO2TOB>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
CLATHRIN-COATED VESICLES; ENDOPLASMIC-RETICULUM; PROTEIN-TRANSPORT; SECRETORY PROTEINS; ACROSOME FORMATION; EXOCRINE PANCREAS; APPARATUS; LOCALIZATION; CELLS; COMPARTMENT;
Keywords:
ACROSOME FORMATION; TGR; COP COAT; CLATHRIN COAT; ENDOPLASMIC RETICULUM GOLGI INTERMEDIATE COMPARTMENT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
J.A. Martinezmenarguez et al., "IDENTIFICATION OF 2 TYPES OF BETA-COP VESICLES IN THE GOLGI-COMPLEX OF RAT SPERMATIDS", European journal of cell biology, 71(2), 1996, pp. 137-143

Abstract

In the present study, immunoelectron microscopy was used to characterize two types of coated vesicles at the trans Golgi reticulum (TGR) ofrat early spermatids. Most of the coated vesicles and buds had a 18.4+/- 0.5 nm thick electron-dense coat. The size of the vesicles without the coat was 102.6 +/- 3.6 nm. This coat reacted with antibodies against clathrin. Immunolabeling for clathrin was almost restricted to the trans Golgi area (86% of the total labeling in the Golgi complex). In addition, we found a homogeneous population of small vesicles and buds bearing a similar to 10 nm thick coat which reacted with antibodiesagainst beta-COP. beta-COP-immunoreactive vesicles were detected at the cis side (32 %), lateral rims (27 %) and trans face (40 %) of the Golgi complex. The diameters of beta-COP-immunoreactive vesicles at theTGR and cis Golgi side were 53.9 +/- 1.3 and 42.1 +/- 1.1 nm, respectively. Cis Golgi elements were identified by using antibodies against markers of the Golgi intermediate compartment p 58, p 53 and Rab 2. Some beta-COP labeling was also found at the acrosomal membrane and associated buds. These results indicate that the TGR of early spermatids contains COP-coated vesicles which are different to those found at the cis Golgi side.

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Documento generato il 04/07/20 alle ore 11:18:20