Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ROLE OF CALCIUM IN STRETCH-INDUCED RELEASE AND MESSENGER-RNA SYNTHESIS OF NATRIURETIC PEPTIDES IN ISOLATED RAT ATRIUM
Autore:
LAINE M; ID L; VUOLTEENAHO O; RUSKOAHO H; WECKSTROM M;
Indirizzi:
UNIV OULU,BIOCTR OULU,DEPT PHYSIOL,KAJAANINTIE 52A SF-90220 OULU FINLAND UNIV OULU,BIOCTR OULU,DEPT PHYSIOL SF-90220 OULU FINLAND UNIV OULU,BIOCTR OULU,DEPT PHARMACOL & TOXICOL SF-90220 OULU FINLAND
Titolo Testata:
Pflugers Archiv
fascicolo: 6, volume: 432, anno: 1996,
pagine: 953 - 960
SICI:
0031-6768(1996)432:6<953:ROCISR>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED ION CHANNELS; VENTRICULAR MYOCYTES; GUINEA-PIG; SECRETION; GADOLINIUM; TISSUE; EXPRESSION; MUSCLE; CA-2+; BLOCK;
Keywords:
ANP; BNP; GADOLINIUM; SECRETION; EXOCYTOSIS; MECHANOTRANSDUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
M. Laine et al., "ROLE OF CALCIUM IN STRETCH-INDUCED RELEASE AND MESSENGER-RNA SYNTHESIS OF NATRIURETIC PEPTIDES IN ISOLATED RAT ATRIUM", Pflugers Archiv, 432(6), 1996, pp. 953-960

Abstract

To investigate the role of Ca2+ in stretch-induced synthesis and release of atrial natriuretic peptide (ANP) and B-type natriuretic peptide(BNP) isolated superfused rat atria were stretched by raising intra-atrial pressure. The immunoreactive (ir-) ANP and BNP concentrations were analysed by radioimmunoassay and the corresponding mRNA levels werequantified by Northern blot and dot blot analyses. Stretch-induced ir-ANP release and a rise in BNP mRNA levels increased at high (3.0 mM) compared to low (0.5 mM) extracellular Ca2+ concentration ([Ca2+](o)). Moreover, the adaptation of stretch-induced ir-ANP release was dependent on [Ca2+](o). Atrial BNP mRNA levels were increased by stretch also in non-paced, electrically silent atria, where voltage-activated Ca2 channels are not activated. The stretch-induced rise in BNP mRNA wasblocked by gadolinium (80 mu M), but not by the L-type channel blocker diltiazem (3.0 mu M). This study indicates that both the stretch-secretion coupling of ir-ANP release and the pressure-stimulated synthesis of BNP mRNA are Ca2+-dependent processes. Gadolinium inhibits the stretch-stimulated rise in BNP mRNA levels in contracting and non-contracting atria, which is similar to its ability to block stretch-activated ir-ANP release, suggesting the involvement of Ca2+-permeable stretch-activated channels.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 12:15:36