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Titolo:
BIOLOGICAL PROPERTIES OF RECOMBINANT CHICKEN INTERFERON-GAMMA
Autore:
WEINING KC; SCHULTZ U; MUNSTER U; KASPERS B; STAEHELI P;
Indirizzi:
UNIV FREIBURG,DEPT VIROL,INST MED MIKROBIOL & HYG,ABT VIROL,HERMANN HERDER STR 11 D-79008 FREIBURG GERMANY UNIV FREIBURG,DEPT VIROL,INST MED MIKROBIOL & HYG,ABT VIROL D-79008 FREIBURG GERMANY UNIV MUNICH,INST TIERPHYSIOL MUNICH GERMANY
Titolo Testata:
European Journal of Immunology
fascicolo: 10, volume: 26, anno: 1996,
pagine: 2440 - 2447
SICI:
0014-2980(1996)26:10<2440:BPORCI>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
EVOLUTIONARY CONSERVATION; MOLECULAR-CLONING; IA-ANTIGEN; EXPRESSION; GENE; SEQUENCE; MHC; CELLS; BETA; MACROPHAGES;
Keywords:
INTERFERON-GAMMA; CHICKEN; CYTOKINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
K.C. Weining et al., "BIOLOGICAL PROPERTIES OF RECOMBINANT CHICKEN INTERFERON-GAMMA", European Journal of Immunology, 26(10), 1996, pp. 2440-2447

Abstract

Supernatants of the chicken T cell line 855 contain antiviral and macrophage-activating factor activity and strongly activate transcriptionof the guanylate-binding protein (GBP) gene in chicken cells. To characterize the cytokine responsible for the GBP-inducing activity, we chose a cDNA expression cloning strategy in COS cells. Sequencing a positive clone revealed that it encodes chicken interferon-gamma (ChIFN-gamma). Histidine-tagged ChIFN-gamma was expressed in Escherichia coli and purified by nickel chelate affinity chromatography. ChIFN-gamma from COS cells and E. coli both potently induced GBP RNA synthesis but were rather poor antiviral agents. In macrophages, recombinant ChIFN-gamma strongly stimulated secretion of nitric oxide and enhanced expression of major histocompatibility complex class II antigen. A rabbit antiserum to E. coli-derived ChIFN-gamma effectively neutralized the macrophage-activating factor activity secreted by concanavalin A-induced spleen cells and various T cell lines, suggesting that IFN-gamma is the major macrophage-activating factor of the chicken.

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Documento generato il 18/01/20 alle ore 13:09:59