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Titolo:
SIMPLE METHOD FOR PRETREATMENT OF TISSUE-SECTIONS FOR THE DETECTION OF APOPTOSIS BY IN-SITU END-LABELING AND IN-SITU NICK TRANSLATION
Autore:
PANCHALINGAM S; REYNOLDS GM; LAMMAS DA; ROWLANDS DC; KUMARARATNE DS;
Indirizzi:
UNIV BIRMINGHAM,SCH MED,DEPT IMMUNOL BIRMINGHAM B15 2TT W MIDLANDS ENGLAND UNIV BIRMINGHAM,SCH MED,DEPT PATHOL BIRMINGHAM B15 2TT W MIDLANDS ENGLAND
Titolo Testata:
JCP. Clinical molecular pathology
fascicolo: 5, volume: 49, anno: 1996,
pagine: 273 - 277
SICI:
1355-2910(1996)49:5<273:SMFPOT>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
DNA STRAND BREAKS; CELL-DEATH; FRAGMENTED DNA; T-CELLS; INSITU; ENDONUCLEASE; NECROSIS; RAT;
Keywords:
APOPTOSIS; IN SITU END-LABELING; IN SITU NICK TRANSLATION; APOPTOSIS SPECIFIC PROTEIN ANTIBODY; PRESSURE COOKER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
S. Panchalingam et al., "SIMPLE METHOD FOR PRETREATMENT OF TISSUE-SECTIONS FOR THE DETECTION OF APOPTOSIS BY IN-SITU END-LABELING AND IN-SITU NICK TRANSLATION", JCP. Clinical molecular pathology, 49(5), 1996, pp. 273-277

Abstract

Aims-To overcome the problems associated with proteolytic pretreatment of tissue sections for the detection of apoptosis. Methods-Formalin fixed, paraffin wax embedded tissue sections of reactive lymph nodes and biopsy specimens of Burkitt lymphoma were pretreated by pressure cooking for the detection of apoptosis using the in situ end-labelling and in situ nick translation methods. Results-The results achieved withthe in situ end-labelling and nick translations methods were comparedwith those obtained using a novel anti-apoptosis specific protein (ASP) antibody. The staining patterns generated using the three methods were similar and consistent, although the ASP antibody seemed to be more sensitive and detected higher numbers of apoptotic cells within sections. Conclusions-Pressure cooking is advocated as an alternative method to proteolytic enzyme digestion for pretreating paraffin wax sections. It is reliable, inexpensive, reduces the need to optimise pretreatment variables for different tissues, and permits double immunostaining of sections.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 07:40:28