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Titolo:
CONTINUOUS ENZYMATIC PRODUCTION OF XYLITOL WITH SIMULTANEOUS COENZYMEREGENERATION IN A CHARGED MEMBRANE REACTOR
Autore:
NIDETZKY B; NEUHAUSER W; HALTRICH D; KULBE KD;
Indirizzi:
AGR UNIV VIENNA,INST FOOD TECHNOL,DIV BIOCHEM ENGN,PETER JORDAN STR 82 A-1190 VIENNA AUSTRIA
Titolo Testata:
Biotechnology and bioengineering
fascicolo: 3, volume: 52, anno: 1996,
pagine: 387 - 396
SICI:
0006-3592(1996)52:3<387:CEPOXW>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
RECOMBINANT SACCHAROMYCES-CEREVISIAE; D-XYLOSE; CANDIDA-TROPICALIS; FORMATE DEHYDROGENASE; GLUCOSE-DEHYDROGENASE; SORBITOL PRODUCTION; ALDOSE REDUCTASE; PURIFICATION; NAD(H); TRANSFORMATION;
Keywords:
XYLITOL; NADH REGENERATION; CHARGED MEMBRANE; CONTINUOUS PRODUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
B. Nidetzky et al., "CONTINUOUS ENZYMATIC PRODUCTION OF XYLITOL WITH SIMULTANEOUS COENZYMEREGENERATION IN A CHARGED MEMBRANE REACTOR", Biotechnology and bioengineering, 52(3), 1996, pp. 387-396

Abstract

We have developed a new process for the production of xylitol from D-xylose by enzyme technology. An NADH-dependent xylose reductase (XR) from Candida tenuis catalyzes the reduction of xylose, which is coupledto enzymatic oxidations of D-glucose or D-xylose by glucose dehydrogenase from Bacillus cereus to make achievable an up to 10,000-fold regeneration of NADH per cycle of discontinuous conversion. Using a simplekinetic model as a tool for process optimization, suitable conditionswith regard to performance and stability of the multicomponent reaction system were established, and 300 g/L of substrate could be converted in yields above 96% in one single batch reaction. Due to selective and over 98% complete retention of the native coenzyme by negatively charged nanofiltration membranes used in a continuously operated enzyme reactor, a specific productivity of 80 g xylitol per liter, day, and kilounit of XR was maintained over the 150-h reaction time with only a single dosage of NADH. (C) 1996 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/09/20 alle ore 10:16:15