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Titolo:
VARIATION IN THE IN-VITRO GENERATED FRUCTAN PATTERN FROM SUCROSE AS AFUNCTION OF THE PURIFIED CHICORY ROOT 1-SST AND 1-FFT CONCENTRATIONS
Autore:
VANDENENDE W; VANLAERE A;
Indirizzi:
KATHOLIEKE UNIV LEUVEN,INST BOT,DEPT BIOL,KARDINAAL MERCIERLAAN 92 B-3001 HEVERLEE BELGIUM KATHOLIEKE UNIV LEUVEN,INST BOT,DEPT BIOL B-3001 HEVERLEE BELGIUM
Titolo Testata:
Journal of Experimental Botany
fascicolo: 304, volume: 47, anno: 1996,
pagine: 1797 - 1803
SICI:
0022-0957(1996)47:304<1797:VITIGF>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXCISED LEAVES; BIOSYNTHESIS; ENZYMES;
Keywords:
1-FFT; 1-SST; CHICORY; ENZYME CONCENTRATION; SUCROSE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
W. Vandenende e A. Vanlaere, "VARIATION IN THE IN-VITRO GENERATED FRUCTAN PATTERN FROM SUCROSE AS AFUNCTION OF THE PURIFIED CHICORY ROOT 1-SST AND 1-FFT CONCENTRATIONS", Journal of Experimental Botany, 47(304), 1996, pp. 1797-1803

Abstract

At low concentrations of purified chicory root 1-SST, only 1-kestose was produced from a physiologically relevant sucrose concentration. Asthe 1-SST concentration increased, some higher oligofructans were also detected, showing that 1-SST has some 1-FFT activity after sucrose exhaustion in the reaction mixtures. The consequences for the interpretation of fructan synthesizing activities in vitro are discussed. With a mixture of both purified 1-SST and 1-FFT it was found that the higher the enzyme concentration, the higher the maximal DP of the fructans that could be synthesized. The higher the enzyme concentration, the higher the relative abundance of the larger DP fructans and fructose in the reaction mixtures, Taken together with previous results, there is confidence that the final fructan pattern obtained in vitro is a function of the (1-SST + 1-FFT)/sucrose ratio and suggest that the latter ratio in situ could affect the highly variable tissue- or species-specific pattern of fructans produced in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 18:11:17