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Titolo:
PRELIMINARY STUDIES ON THE CRYOPRESERVATION OF RED-BLOOD-CELLS OF RAINBOW-TROUT (ONCORHYNCHUS-MYKISS) AND EUROPEAN CATFISH (SILURUS-GLANIS L)
Autore:
ZHANG TT; MAISSE G; RAWSON DM;
Indirizzi:
UNIV LUTON,RES CTR,24 CRAWLEY GREEN RD LUTON LU1 3LF BEDS ENGLAND INRA,PHYSIOL POISSONS LAB F-35042 RENNES FRANCE
Titolo Testata:
Cryo-letters
fascicolo: 5, volume: 17, anno: 1996,
pagine: 303 - 308
SICI:
0143-2044(1996)17:5<303:PSOTCO>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOTOXICITY; ASSAYS; LINE;
Keywords:
CRYOPRESERVATION; RED BLOOD CELLS; RAINBOW TROUT (ONCORHYNCHUS MYKISS); EUROPEAN CATFISH (SILURUS GLANIS L); DMSO; CONTROLLED SLOW COOLING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
13
Recensione:
Indirizzi per estratti:
Citazione:
T.T. Zhang et al., "PRELIMINARY STUDIES ON THE CRYOPRESERVATION OF RED-BLOOD-CELLS OF RAINBOW-TROUT (ONCORHYNCHUS-MYKISS) AND EUROPEAN CATFISH (SILURUS-GLANIS L)", Cryo-letters, 17(5), 1996, pp. 303-308

Abstract

Cryopreservation of red blood cells of rainbow trout (Oncorhynchus mykiss) and European catfish (Silurus glanis L.) was studied using controlled slow cooling. Dimethyl sulfoxide (DMSO) was found to be the least toxic and most effective cryoprotectant when compared with methanol,glycerol and propan-1,2-diol. The best recoveries of intact cells, achieved after cryopreservation under the selected conditions, were 86.3+/- 8.1 and 89.7 +/- 7.9 for rainbow trout and catfish respectively. These results open up the possibility of cryopreserved fish red blood cells being used in cytotoxic assays.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 11:21:54