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Titolo:
ADVENTITIOUS SHOOT REGENERATION FROM FAGUS-SYLVATICA LEAF EXPLANTS IN-VITRO
Autore:
VIEITEZ AM; SANJOSE MC;
Indirizzi:
CSIC,INST INVEST AGROBIOL GALICIA,APARTADO 122 SANTIAGO COMPOSTE 15080 SPAIN
Titolo Testata:
In vitro cellular & developmental biology. Plant
fascicolo: 3, volume: 32, anno: 1996,
pagine: 140 - 147
SICI:
1054-5476(1996)32:3<140:ASRFFL>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
ASH FRAXINUS-EXCELSIOR; SOMATIC EMBRYOGENESIS; APPLE ROOTSTOCK; EXCISED LEAVES; INVITRO; THIDIAZURON; SEGMENTS; CULTURE; PLANTS; MICROPROPAGATION;
Keywords:
ADVENTITIOUS BUDS; EUROPEAN BEECH; LEAF CULTURE; SHOOT REGENERATION; THIDIAZURON;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
A.M. Vieitez e M.C. Sanjose, "ADVENTITIOUS SHOOT REGENERATION FROM FAGUS-SYLVATICA LEAF EXPLANTS IN-VITRO", In vitro cellular & developmental biology. Plant, 32(3), 1996, pp. 140-147

Abstract

Adventitious shoots were induced on transversally divided expanding leaves from Fagus sylvatica shoot cultures of juvenile origin. Adventitious shoot buds formed mainly on callus that developed on the petiole stump or on the cut across the midrib of distal leaf halves. However, sometimes they arose directly from leaf tissue. An anatomical study confirmed both the direct and indirect origin of the adventitious buds. The best results were obtained by culturing proximal leaf sections on woody plant medium supplemented with 2.9 mu M indole-3 acetic acid in combination with 8.9 mu M benzyladenine or 2.3 mu M thidiazuron (TDZ). Proximal explants were more responsive than distal explants in terms of both callus formation and bud regeneration, regardless of the induction medium or clone tested. Bud formation capacity was influenced by the genotype of the stock shoot culture and was enhanced by an initial10 d darkness, but was inhibited by longer periods of darkness. Caulogenic competence was significantly affected by the duration of exposure to TDZ; in particular, adventitious shoot length was depressed by increasing the exposure period. Three weeks culture with TDZ was the most efficient treatment for shoot production and elongation. Further shoot development was promoted by subculturing the explants to the same medium used for the maintenance of the stock shoot cultures. Shoots so obtained were multiplied and rooted producing plantlets of adventitious origin.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 12:11:05