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Titolo:
RAPD ANALYSIS OF LATHYRUS-SATIVUS SOMACLONES
Autore:
MANDAL PK; SANTHA IM; MEHTA SL;
Indirizzi:
INDIAN AGR RES INST,DIV BIOCHEM NEW DELHI 110012 INDIA INDIAN AGR RES INST,DIV BIOCHEM NEW DELHI 110012 INDIA
Titolo Testata:
Journal of Plant Biochemistry and Biotechnology
fascicolo: 2, volume: 5, anno: 1996,
pagine: 83 - 86
SICI:
0971-7811(1996)5:2<83:RAOLS>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLANT-REGENERATION; EXPLANTS; POTATO;
Keywords:
LATHYRUS SATIVUS; SOMACLONES; RAPD; DNA FINGERPRINTING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
P.K. Mandal et al., "RAPD ANALYSIS OF LATHYRUS-SATIVUS SOMACLONES", Journal of Plant Biochemistry and Biotechnology, 5(2), 1996, pp. 83-86

Abstract

RAPD patterns were studied in seven somaclones of Lathyrus sativus having contrasting characteristics along with the parent cultivar P-24. Out of 81 decamer random primers used, 5 did not amplify and 24 revealed DNA polymorphism while the rest generated monomorphic banding patterns. Eight unique bands were amplified with different primers in four different somaclones. With most of the informative primers differenceswere observed between somaclones and also between some of the somaclones and parent cultivar P-24. More than 90% similarity in the RAPD patterns was evident among the somaclones and the parent cultivar P-24. Though it was not possible to identify a particular somaclone with a single primer, a combination of two or more primers could be employed toidentify a somaclone.

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Documento generato il 28/11/20 alle ore 14:06:10