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Titolo:
EXPRESSION OF MOUSE METALLOTHIONEIN IN THE CYANOBACTERIUM SYNECHOCOCCUS PCC7942
Autore:
ERBE JL; TAYLOR KB; HALL LM;
Indirizzi:
UNIV ALABAMA,DEPT BIOCHEM & MOL GENET,933 SOUTH 19TH ST BIRMINGHAM AL35294 UNIV ALABAMA,DEPT BIOCHEM & MOL GENET BIRMINGHAM AL 35294
Titolo Testata:
Journal of industrial microbiology
fascicolo: 1, volume: 17, anno: 1996,
pagine: 41 - 46
SICI:
0169-4146(1996)17:1<41:EOMMIT>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALCALIGENES-EUTROPHUS CH34; ANACYSTIS-NIDULANS R2; ESCHERICHIA-COLI; PROKARYOTIC METALLOTHIONEIN; HEAVY-METALS; GENE CLONING; I GENE; CADMIUM; LOCUS; RESISTANCE;
Keywords:
METALLOTHIONEIN; CYANOBACTERIA; METALS; BIOREMEDIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
J.L. Erbe et al., "EXPRESSION OF MOUSE METALLOTHIONEIN IN THE CYANOBACTERIUM SYNECHOCOCCUS PCC7942", Journal of industrial microbiology, 17(1), 1996, pp. 41-46

Abstract

A cDNA encoding mouse metallothionein was cloned into the shuttle vector pUC303, creating a translational fusion with the bacterial chloramphenicol acetyltransferase gene. The resulting fusion protein has beenexpressed in the cyanobacterium Synechococcus PCC7942. Cyanobacterialtransformants expressed mouse metallothionein-specific mRNA species as detected by RNA slot blots. In addition, the transformants expresseda unique cadmium ion-binding protein corresponding to the predicted size of the mouse metallothionein fusion protein. Expression of this fusion protein conferred a two- to five-fold increase in cadmium ion tolerance and accumulation on Synechococcus PCC7942.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 20:39:18