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Titolo:
A COMPARATIVE-STUDY OF THE BROTH MICRO-DILUTION AND MACRO-DILUTION TECHNIQUES FOR THE DETERMINATION OF THE IN-VITRO SUSCEPTIBILITY OF ASPERGILLUS-FUMIGATUS
Autore:
MANAVATHU EK; ALANGADEN GJ; LERNER SA;
Indirizzi:
WAYNE STATE UNIV,DEPT INTERNAL MED,DIV INFECT DIS,427 LANDE BLDG,550 E CANFIELD DETROIT MI 48201
Titolo Testata:
Canadian journal of microbiology
fascicolo: 9, volume: 42, anno: 1996,
pagine: 960 - 964
SICI:
0008-4166(1996)42:9<960:ACOTBM>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACQUIRED-IMMUNODEFICIENCY-SYNDROME; BONE-MARROW TRANSPLANTATION; INVASIVE ASPERGILLOSIS; PULMONARY ASPERGILLOSIS; FILAMENTOUS FUNGI; ACUTE-LEUKEMIA; AIDS; OSTEOMYELITIS; ANTIFUNGAL; MACRODILUTION;
Keywords:
ASPERGILLUS FUMIGATUS; SUSCEPTIBILITY TEST; ANTIFUNGALS; DRUG RESISTANCE; BROTH MACRODILUTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
E.K. Manavathu et al., "A COMPARATIVE-STUDY OF THE BROTH MICRO-DILUTION AND MACRO-DILUTION TECHNIQUES FOR THE DETERMINATION OF THE IN-VITRO SUSCEPTIBILITY OF ASPERGILLUS-FUMIGATUS", Canadian journal of microbiology, 42(9), 1996, pp. 960-964

Abstract

The effects of inoculum size, medium, temperature, anti duration of growth on the in vitro susceptibility testing of Aspergillus fumigatus were investigated using broth micro- and macro-dilution techniques. The minimum inhibitory concentrations (MICs) of ketoconazole, miconazole, itraconazole, fluconazole, and amphotericin B were significantly influenced by the inoculum size, regardless of the techniques used. Two- to four-fold higher MIC values were obtained when the inoculum size was increased 100-fold. The use of peptone yeast extract glucose and RPMI 1640 media provided essentially identical MIC values at 30 and 35 degrees C after incubation for 48 h or longer. A comparison of broth micro- and macro-dilution techniques revealed that, under equivalent conditions, the latter with an inoculum size between 1 x 10(3) and 1 x 10(4) conidia (strain W73355)/mL consistently provided the lowest MICs offluconazole (256 mu g/mL), ketoconazole (8 mu g/mL), miconazole (2 mug/mL), itraconazole (0.25 mu g/mL), and amphotericin B (0.25 mu g/mL). Using the broth macrodilution technique. we screened 24 clinical isolates of A. fumigatus obtained from the Detroit Medical Center in 1994. The MIC values of fluconazole, ketoconazole, miconazole, itraconazole and amphotericin B for all the isolates were 128-256, 8-16, 1-2, 0.25-0.5, and 0.25-1.0 mu g/mL, respectively, indicating that none of theclinical isolates that we tested shows acquired resistance to the antifungals used.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 10:23:54