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Titolo:
PREPARATION OF MYRISTOYLATED AND NONMYRIS TOYLATED RECOMBINANT RECOVERINS IN ESCHERICHIA-COLI AND COMPARISON OF THEIR FUNCTIONAL ACTIVITIES
Autore:
ZARGAROV AA; SENIN II; ALEKSEEV AM; SHULGAMORSKOI SV; PHILIPPOV PP; LIPKIN VM;
Indirizzi:
RUSSIAN ACAD SCI,SHEMYAKIN OCHINNIKOV INST BIOORGAN CHEM,PUSHCHINO BRANCH PUSHCHINO 142292 MOSCOW OBLAST RUSSIA MOSCOW MV LOMONOSOV STATE UNIV,BELOZERSKY INST PHYSICOCHEM BIOL MOSCOW 119899 RUSSIA
Titolo Testata:
Bioorganiceskaa himia
fascicolo: 7, volume: 22, anno: 1996,
pagine: 483 - 488
SICI:
0132-3423(1996)22:7<483:POMANT>2.0.ZU;2-4
Fonte:
ISI
Lingua:
RUS
Soggetto:
CALCIUM-BINDING PROTEIN; ROD OUTER SEGMENTS; RHODOPSIN PHOSPHORYLATION; GUANYLATE-CYCLASE; S-MODULIN; CELLS; MEMBRANE; KINASE; PURIFICATION; ACTIVATOR;
Keywords:
RECOVERIN; PHOTORECEPTION; PHOSPHORYLATION; RHODOPSIN; RHODOPSIN KINASE; CALCIUM-BINDING PROTEINS; EXPRESSION IN ESCHERICHIA-COLI;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
A.A. Zargarov et al., "PREPARATION OF MYRISTOYLATED AND NONMYRIS TOYLATED RECOMBINANT RECOVERINS IN ESCHERICHIA-COLI AND COMPARISON OF THEIR FUNCTIONAL ACTIVITIES", Bioorganiceskaa himia, 22(7), 1996, pp. 483-488

Abstract

A recombinant plasmid was constructed for expressing a gene for bovine recoverin under the control of the lac promoter. Coexpression of therecoverin and N-myristoyl transferase genes was performed to prepare recombinant myristoylated recoverin. The obtained systems provide highlevels of biosynthesis of the recombinant recoverins in the E. coli cells. Using a reconstructed system, containing urea washed rod outer segment membranes, purified rhodopsin kinase (RK), and a recoverin, it was shown that the three recoverin forms (natural, recombinant nonmyristoylated, and recombinant myristoylated ones) perform the calcium-dependent regulation of the activity of RK with half a maximum effect at a free calcium concentration of 2 mu M. Interestingly, the N-terminal myristoylation of recoverin increased substantially its functional activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 01:54:01