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Titolo:
CROSS-LINKING OF THE HIGH-AFFINITY IGE RECEPTOR INDUCES THE EXPRESSION OF CYCLOOXYGENASE-2 AND ATTENDANT PROSTAGLANDIN GENERATION REQUIRINGINTERLEUKIN-10 AND INTERLEUKIN-1-BETA IN MOUSE CULTURED MAST-CELLS
Autore:
ASHRAF M; MURAKAMI M; KUDO I;
Indirizzi:
SHOWA UNIV,SCH PHARMACEUT SCI,DEPT HLTH CHEM,SHINAGAWA KU,1-5-8 HATANODAI TOKYO 142 JAPAN SHOWA UNIV,SCH PHARMACEUT SCI,DEPT HLTH CHEM,SHINAGAWA KU TOKYO 142 JAPAN
Titolo Testata:
Biochemical journal
, volume: 320, anno: 1996,
parte:, 3
pagine: 965 - 973
SICI:
0264-6021(1996)320:<965:COTHIR>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
C-KIT LIGAND; CYTOSOLIC PHOSPHOLIPASE A(2); BASOPHILIC LEUKEMIA-CELLS; ENDOPEROXIDE SYNTHASE-1; HISTAMINE-RELEASE; G/H SYNTHASE; TYROSINE PHOSPHORYLATION; LEUKOTRIENE C-4; D-2 GENERATION; RAT;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
67
Recensione:
Indirizzi per estratti:
Citazione:
M. Ashraf et al., "CROSS-LINKING OF THE HIGH-AFFINITY IGE RECEPTOR INDUCES THE EXPRESSION OF CYCLOOXYGENASE-2 AND ATTENDANT PROSTAGLANDIN GENERATION REQUIRINGINTERLEUKIN-10 AND INTERLEUKIN-1-BETA IN MOUSE CULTURED MAST-CELLS", Biochemical journal, 320, 1996, pp. 965-973

Abstract

When mouse bone marrow-derived mast cells (BMMC) developed in interleukin (IL)-3 were activated with IgE and antigen (IgE/antigen) in the presence of both IL-10 and IL-1 beta, two sequential phases of prostaglandin (PG)D-2 generation were elicited, in which the first phase occurred by 1 h and the second phase from 2 to 10 h. The delayed phase of PGD(2) generation was accompanied by a marked induction of cyclo-oxygenase (COX)-2 mRNA, which reached a peak at 1-2 h, followed by that of its protein from 2-10 h, with a peak at 5 h. The immediate phase of PGD(2) generation was completely abrogated by the irreversible inhibitionof pre-exisiting COX-1 by aspirin pretreatment, whereas the delayed phase of PGD(2) generation was almost undetectable in the presence of the COX-2 inhibitor NS-398. A detailed analysis of the individual effects of IgE/antigen, IL-10 and IL-1 beta on COX-2 expression revealed that IgE/antigen and IL-10 each initiated and stabilized COX-2 mRNA expression, leading to an increase in the expression of its protein. Conversely, IL-1 beta stabilized the COX-2 protein without affecting its mRNA level. The induction of COX-2 by IgE/antigen with IL-10 and IL-1 beta preceded the induction of transcripts for endogenous cytokines suchas IL-6, IL-1 beta and IL-10. The inhibition of PGD(2) generation by indomethacin did not affect the induction of COX-2 or these cytokines. Thus the two major delayed-phase responses of BMMC after IgE-dependent activation, namely COX-2-dependent PGD(2) generation and cytokine production, are regulated independently.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 07:17:45