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Titolo:
CONTINUOUS RETENTION OF NATIVE NADP(H) IN AN ENZYME MEMBRANE REACTOR FOR GLUCONATE AND GLUTAMATE PRODUCTION
Autore:
OBON JM; ALMAGRO MJ; MANJON A; IBORRA JL;
Indirizzi:
UNIV MURCIA,FAC QUIM,DEPT BIOQUIM & BIOL MOL B IMMUNOL,APDO CORREOS 4021 MURCIA 30001 SPAIN UNIV MURCIA,FAC QUIM,DEPT BIOQUIM & BIOL MOL B IMMUNOL MURCIA 30001 SPAIN
Titolo Testata:
Journal of biotechnology
fascicolo: 1, volume: 50, anno: 1996,
pagine: 27 - 36
SICI:
0168-1656(1996)50:1<27:CRONNI>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
COENZYME REGENERATION SYSTEM; SORBITOL PRODUCTION; BIOREACTOR; NAD+;
Keywords:
NADP(H) RETENTION; COENZYME REGENERATION; COUPLED REACTIONS; GLUCOSE DEHYDROGENASE; GLUTAMATE DEHYDROGENASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
J.M. Obon et al., "CONTINUOUS RETENTION OF NATIVE NADP(H) IN AN ENZYME MEMBRANE REACTOR FOR GLUCONATE AND GLUTAMATE PRODUCTION", Journal of biotechnology, 50(1), 1996, pp. 27-36

Abstract

A new concept of membrane reactor for continuous retention of NADP(H)with non-charged ultrafiltration membranes is proposed. The presence of the high molecular weight polymer polyethyleneimine (PEI) in the reaction medium allow to achieve high retainment ratios for the native coenzyme when the molar ratio PEI/NADP(H) in the reactor is >1. NaCl concentrations >0.3 M decrease significantly the retainment ratio of NADP(+) in the reactor. Therefore, this system is applicable for enzyme reactions requiring low ionic strength media. This reactor configuration has been applied to obtain simultaneously gluconate and glutamate bycoupling two enzymes for coenzyme regeneration, glucose dehydrogenaseand glutamate dehydrogenase. Sorbitol stabilization of the halophilicenzymes allows to compensate the reduced NaCl concentration required for coenzyme retention. Experimental performance of the reaction is compared successfully with a theoretical model that considers the kinetic mechanisms of the enzymes involved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 23:50:15