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Titolo:
THE LTR, V-SRC, LTR PROVIRUS IN H-19 HAMSTER TUMOR-CELL LINE IS INTEGRATED ADJACENT TO THE NEGATIVE REGULATORY REGION
Autore:
MACHON O; HEJNAR J; HAJKOVA P; GERYK J; SVOBODA J;
Indirizzi:
ACAD SCI CZECH REPUBL,INST MOL GENET,FLEMINGOVO NAM 2 CZ-16637 PRAGUE6 CZECH REPUBLIC ACAD SCI CZECH REPUBL,INST MOL GENET CZ-16637 PRAGUE 6 CZECH REPUBLIC
Titolo Testata:
Gene
fascicolo: 1, volume: 174, anno: 1996,
pagine: 9 - 17
SICI:
0378-1119(1996)174:1<9:TLVLPI>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
ROUS-SARCOMA PROVIRUSES; TRANSFORMED RAT-CELLS; TRANSCRIPTIONAL SUPPRESSION; EXPRESSION; SEQUENCES; GENOME; GENE;
Keywords:
RETROVIRAL INTEGRATION; GC CONTENT; SILENCER; CPG ISLAND; DNA METHYLATION; DNA-PROTEIN INTERACTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
O. Machon et al., "THE LTR, V-SRC, LTR PROVIRUS IN H-19 HAMSTER TUMOR-CELL LINE IS INTEGRATED ADJACENT TO THE NEGATIVE REGULATORY REGION", Gene, 174(1), 1996, pp. 9-17

Abstract

The tumor hamster cell line H-19 harbors a single copy LTR, v-src, LTR provirus that becomes permanently transcriptionally suppressed in morphological revertants segregating at high rate from this cell line. Our previous data document that the provirus suppression is mediated byepigenetic cell-regulatory mechanisms. In this report, we concentrateon cellular sequences neighboring the integration site. The locus is unique for Syrian hamster and is not detectable in DNA of several animal species. No restriction sites that usually hint at the presence of CpG islands were found in the significantly close vicinity of the provirus. Nevertheless, the chromosomal DNA flanking the provirus is rich in GC content (57.8%). We localized a 0.5-kb region downstream from the provirus that remarkably inhibits transcription in the transient expression assay and is effective both on the homologous RSV LTR promoter/enhancer and heterologous SV40 promoter. We propose that a cellular trans-acting factor is involved in the silencing of the reporter gene. Since this activity is comparable both in transformed and revertant cells, we speculate that this down-regulatory region makes the permissive integration locus prone to provirus silencing initiated by other fluctuating stimuli.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 00:15:49