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Titolo:
HOXB CLUSTER GENES IN ACTIVATED NATURAL-KILLER LYMPHOCYTES EXPRESSIONFROM 3'-]5'-CLUSTER SIDE AND PROLIFERATIVE FUNCTION
Autore:
QUARANTA MT; PETRINI M; TRITARELLI E; SAMOGGIA P; CARE A; BOTTERO L; TESTA U; PESCHLE C;
Indirizzi:
THOMAS JEFFERSON UNIV,THOMAS JEFFERSON CANC INST,233 S 10TH ST PHILADELPHIA PA 19107 THOMAS JEFFERSON UNIV,THOMAS JEFFERSON CANC INST PHILADELPHIA PA 19107 IST SUPER SANITA,DEPT HEMATOL ONCOL SANITA ITALY
Titolo Testata:
The Journal of immunology
fascicolo: 6, volume: 157, anno: 1996,
pagine: 2462 - 2469
SICI:
0022-1767(1996)157:6<2462:HCGIAN>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
EMBRYONAL CARCINOMA-CELLS; HUMAN HEMATOPOIETIC-CELLS; NK-TR GENE; HOMEOBOX GENE; RETINOIC ACID; PROGENITOR DIFFERENTIATION; SEQUENTIAL ACTIVATION; COORDINATE EXPRESSION; LEUKEMIA; INHIBITION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
M.T. Quaranta et al., "HOXB CLUSTER GENES IN ACTIVATED NATURAL-KILLER LYMPHOCYTES EXPRESSIONFROM 3'-]5'-CLUSTER SIDE AND PROLIFERATIVE FUNCTION", The Journal of immunology, 157(6), 1996, pp. 2462-2469

Abstract

The expression of HOXB cluster genes (i.e., B7 through B9) was evaluated in purified IL-2/1L-1 beta B-activated NK lymphocytes from normal adult peripheral blood by RNase protection and reverse transcription-PCR. In quiescent NK cells these genes are essentially not expressed. After lL-2/lL-1 beta addition, we observed a coordinate induction wave in the 3'-->5' HOXB cluster direction, i.e., from B1 through B9. As notable exceptions, B8 is silent, while B9 RNA is detected starting from6 h through day 11. Furthermore, the 3' located B2/B3/B4 are expressed earlier and at higher level than the 5' located B5/B6/B7/B8. In lL-2/IL-1 beta-activated NK cells, treatment with antisense oligonucleotides targeting B2 mRNA causes a significant inhibition of both cell proliferation and expression of activation markers (i.e., IL-2R alpha-chain and transferrin receptor). These studies provide novel evidence of the role of HOX genes in adult NK cell proliferation. Thus, 1) a coordinate activation of HOXB genes from the 3'-->5' cluster side apparentlyunderlies Ib-2/IL-1 beta-induced NK cell activation. 2) Since NK cellactivation and survival induced by IL-12 and c-kif ligand, respectively, are not associated with cell proliferation of HOXB gene expression, it is apparent that HOXB gene induction is specifically associated with IL-2-induced NK cell proliferation. 3) Studies with antisense oligomer targeting HOXB2 mRNA suggest an important role for B2 in NK cell proliferation, possibly in part via the IL-2R.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 20:19:25