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Titolo:
AUTOCRINE LOOP THROUGH CHOLECYSTOKININ-B GASTRIN RECEPTORS INVOLVED IN GROWTH OF HUMAN LEUKEMIA-CELLS/
Autore:
IWATA N; MURAYAMA T; MATSUMORI Y; ITO M; NAGATA A; TANIGUCHI T; CHIHARA K; MATSUO Y; MINOWADA J; MATSUI T;
Indirizzi:
KOBE UNIV,SCH MED,DEPT MED,DIV 3,CHUO KU,KUSUNOKI CHO KOBE 650 JAPAN KOBE UNIV,SCH MED,DEPT MED,DIV 3,CHUO KU KOBE 650 JAPAN HAYASHIBARA BIOCHEM LABS INC,FUJISAKI CELL CTR OKAYAMA JAPAN
Titolo Testata:
Blood
fascicolo: 7, volume: 88, anno: 1996,
pagine: 2683 - 2689
SICI:
0006-4971(1996)88:7<2683:ALTCGR>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
B GASTRIN RECEPTOR; HUMAN-MONOCYTES; MEDIATED CHEMOTAXIS; FUNCTIONAL-CHARACTERIZATION; SUBSTANCE-P; GENE; EXPRESSION; PEPTIDE; BRAIN; LOCALIZATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
N. Iwata et al., "AUTOCRINE LOOP THROUGH CHOLECYSTOKININ-B GASTRIN RECEPTORS INVOLVED IN GROWTH OF HUMAN LEUKEMIA-CELLS/", Blood, 88(7), 1996, pp. 2683-2689

Abstract

The cholecystokinin (CCK)-B/gastrin receptor binds two brain-gut hormones, CCK and gastrin, with high affinities. These peptides have a trophic effect on gastrointestinal cells expressing the receptor in vivo as well as in vitro. Recently, this receptor mRNA was reported to be expressed in immunocytes localized in the lamina propria of normal rat stomach mucose. Here, we studied the receptor expression in human hematopoietic cells in order to determine whether they play a role in cellgrowth. The CCK-B/gastrin receptor mRNA was detectable in the polymorphonuclear (PMN) cells but not in the mononuclear cells of normal peripheral white blood cells by reverse transcription-polymerase chain reaction. The receptor transcript was, however, expressed in human leukemia cell lines (14 of 18 cell lines tested) derived from not only myeloid, but also T- and B- lymphoid lineages. The CCK-B/gastrin receptors on several leukemia cell lines were shown to be biologically active bydemonstrating ligand-dependent cell proliferation in serum-deprived medium. Interestingly, a human CCK-B/gastrin receptor specific antagonist, YM022, but not its stereotype isoform, selectively inhibited the DNA synthesis of THP-1, MOLT-II, MOLT-14, and CCRF-CEM in the absence of exogenous peptide ligands. Further investigation revealed that theseleukemia cell lines and normal PMN cells also expressed gastrin mRNA. These results suggest that growth of human leukemia cells is promotedby an autocrine mechanism through the CCK-B/gastrin receptors. (C) 1996 by The American Society of Hematology.

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Documento generato il 27/11/20 alle ore 16:07:40