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Titolo:
DIFFERENTIAL BINDING AVIDITIES OF HUMAN-IGM FOR STAPHYLOCOCCAL PROTEIN-A DERIVE FROM SPECIFIC GERM-LINE VH3 GENE USAGE
Autore:
HAKODA M; KAMATANI N; HAYASHIMOTOKURUMADA S; SILVERMAN GJ; YAMANAKA H; TERAI C; KASHIWAZAKI S;
Indirizzi:
TOKYO WOMENS MED COLL,INST RHEUMATOL,SHINJUKU KU,KS BLDG,9-12 WAKAMATSU CHO TOKYO 162 JAPAN UNIV CALIF SAN DIEGO,DEPT MED LA JOLLA CA 92093
Titolo Testata:
The Journal of immunology
fascicolo: 7, volume: 157, anno: 1996,
pagine: 2976 - 2981
SICI:
0022-1767(1996)157:7<2976:DBAOHF>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
SYNOVIAL TISSUE; B-CELLS; RHEUMATOID-ARTHRITIS; VARIABLE REGIONS; AUREUS; CHAIN; RECOGNITION; LYMPHOCYTES; REPERTOIRE; RECEPTORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
M. Hakoda et al., "DIFFERENTIAL BINDING AVIDITIES OF HUMAN-IGM FOR STAPHYLOCOCCAL PROTEIN-A DERIVE FROM SPECIFIC GERM-LINE VH3 GENE USAGE", The Journal of immunology, 157(7), 1996, pp. 2976-2981

Abstract

Human IgM that express the variable region genes of the VH3 family bind staphylococcal protein A (SPA), We previously reported that the SPA-binding IgM can be divided into two groups based on the differential binding avidities for solid-phase SPA, To study the molecular basis for these differences, we cloned B cells from human blood by EBV transformation, The nucleotide sequences of the expressed Ig heavy chain genes were determined on 20 B cell clones that produce SPA-binding IgM, The germ-line VH3 gene usage in IgM with high avidities for SPA were distinct from the germ-line VH3 genes used in IgM with low avidities for SPA. There was no correlation in the usage of D or J(H) genes or in the usage of light chains in IgM according to the SPA binding avidity, These results suggest that the differential binding avidities for SPA are at least partly due to specific germ-line VH3 gene usage. An investigation of direct binding of SPA to the synthetic peptides corresponding to the portions of the variable regions of SPA-binding and non-SPA-binding IgM showed that the peptides corresponding to the VH3 family specific framework region 3 sequences had significant SPA binding capacities, while the peptides corresponding to the other subdomains and those corresponding to framework region 3 of the reported VH3 sequences from non-SPA-binding IgM showed little or no binding, It is of interest that the Ig framework region 3 subdomain corresponds to the fourth hypervariable region,which in the TCR-beta chain has been implicated asa critical site for T cell superantigen binding.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 16:05:07