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Titolo:
HEPATIC NUCLEOTIDE TRIPHOSPHATE REGENERATION AFTER HYPOTHERMIC REPERFUSION IN THE PIG MODEL - AN IN-VITRO P-31-NMR STUDY
Autore:
CHANGANI KK; FULLER BJ; BELL JD; BRYANT DJ; MOORE DP; TAYLORROBINSON SD; DAVIDSON BR;
Indirizzi:
ROYAL FREE HOSP,SCH MED,DEPT SURG,ROWLAND HILL ST LONDON NW3 2QG ENGLAND ROYAL FREE HOSP,SCH MED,LIVER TRANSPLANT UNIT LONDON NW3 2QG ENGLAND HAMMERSMITH HOSP,ROYAL POSTGRAD MED SCH,DIV GASTROENTEROL LONDON W12 0NN ENGLAND HAMMERSMITH HOSP,ROYAL POSTGRAD MED SCH,ROBERT STEINER NMR UNIT LONDON W12 0NN ENGLAND
Titolo Testata:
Transplantation
fascicolo: 6, volume: 62, anno: 1996,
pagine: 787 - 793
SICI:
0041-1337(1996)62:6<787:HNTRAH>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEAR-MAGNETIC-RESONANCE; RAT-LIVER; UW SOLUTION; ADENINE-NUCLEOTIDES; PRESERVATION; VIABILITY; LACTOBIONATE; METABOLITES; ISCHEMIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
K.K. Changani et al., "HEPATIC NUCLEOTIDE TRIPHOSPHATE REGENERATION AFTER HYPOTHERMIC REPERFUSION IN THE PIG MODEL - AN IN-VITRO P-31-NMR STUDY", Transplantation, 62(6), 1996, pp. 787-793

Abstract

The aim of this study was to assess the possibility of regenerating nucleotide triphosphates (NTP) in the pig liver following its harvest and subsequent storage on ice. This study has used a pig model that allowed human donor liver retrieval techniques and methods of storage to be utilized, In vitro phosphorus-31 nuclear magnetic resonance (P-31-NMR) spectroscopy was used to evaluate the changes associated with phosphorus containing metabolites such as NTP, phosphomonoesters (PME), phosphodiesters (PDE), and inorganic phosphate (Pi), During 4 hr storageNTP levels were reduced to undetectable levels but its regeneration was possible over a period of 2 hr of oxygenated hypothermic reperfusion, Resynthesized NTP reached values that were only 30% reduced from preharvest values, There was a corresponding reduction in Pi over the same period, Glycolytic intermediates, 3-phosphoglycerate and 2,3 diphosphoglycerate, both increased significantly during the period of storage and subsequently declined following hypothermic reperfusion, Cellular damage, indicated by the concentrations of glycerophosphorylcholine (GPC) and glycerophosphorylethanolamine (GPE) was minimal during cold storage, However upon hypothermic reperfusion, concentrations of GPC and GPE reduced, indicating a degree of cellular damage caused by reperfusion, This study has shown for the first time that it is possible toregenerate high energy phosphate nucleotides following a period of hypothermic reperfusion in a large, clinically related animal model. This technique warrants investigation clinically to improve the outcome of orthotopic liver transplantation. It also provides a method to studythe effects of different preservation fluids and methods of storage and organ reperfusion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 10:40:22