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Titolo:
POLY(RC) BINDING-PROTEIN-2 BINDS TO STEM-LOOP-IV OF THE POLIOVIRUS RNA 5'-NONCODING REGION - IDENTIFICATION BY AUTOMATED LIQUID-CHROMATOGRAPHY TANDEM MASS-SPECTROMETRY
Autore:
BLYN LB; SWIDEREK KM; RICHARDS O; STAHL DC; SEMLER BL; EHRENFELD E;
Indirizzi:
UNIV CALIF IRVINE,DEPT MOL BIOL & BIOCHEM IRVINE CA 92717 UNIV CALIF IRVINE,DEPT MICROBIOL & MOL GENET IRVINE CA 92717 CITY HOPE NATL MED CTR,BECKMAN RES INST,DIV IMMUNOL DUARTE CA 91010
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 20, volume: 93, anno: 1996,
pagine: 11115 - 11120
SICI:
0027-8424(1996)93:20<11115:PBBTSO>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENCEPHALOMYOCARDITIS VIRUS-RNA; INTERNAL RIBOSOMAL ENTRY; 5'-NONCODING REGION; TRANSLATION; INITIATION; INVOLVEMENT; COMPONENT; SEQUENCES; ELEMENTS; COMPLEX;
Keywords:
POLIOVIRUS; TRANSLATION; POLY(RC) BINDING PROTEIN 2; PROTEIN SEQUENCE ANALYSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
L.B. Blyn et al., "POLY(RC) BINDING-PROTEIN-2 BINDS TO STEM-LOOP-IV OF THE POLIOVIRUS RNA 5'-NONCODING REGION - IDENTIFICATION BY AUTOMATED LIQUID-CHROMATOGRAPHY TANDEM MASS-SPECTROMETRY", Proceedings of the National Academy of Sciences of the United Statesof America, 93(20), 1996, pp. 11115-11120

Abstract

The 5' noncoding region of poliovirus RNA contains an internal ribosome entry site (IRES) for cap-independent initiation of translation, Utilization of the IRES requires the participation of one or more cellular proteins that mediate events in the translation initiation reaction, but whose biochemical roles have not been defined, In this report, we identify a cellular RNA binding protein isolated from the ribosomal salt wash of uninfected HeLa cells that specifically binds to stem-loop IV, a domain located in the central part of the poliovirus IRES, Theprotein was isolated by specific RNA affinity chromatography, and 55%of its sequence was determined by automated liquid chromatography-tandem mass spectrometry. The sequence obtained matched that of poly(rC) binding protein 2 (PCBP2), previously identified as an RNA binding protein from human cells, PCBP2, as well as a related protein, PCBP1, wasover-expressed in Escherichia coli after cloning the cDNAs into an expression plasmid to produce a histidine-tagged fusion protein, Specific interaction between recombinant PCBP2 and poliovirus stem-loop IV was demonstrated by RNA mobility shift analysis, The closely related PCBP1 showed no stable interaction with the RNA, Stem-loop IV RNA containing a three nucleotide insertion that abrogates translation activity and virus viability was unable to bind PCBP2.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 06:46:12