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Titolo:
ACTIVATION DOMAIN-MEDIATED ENHANCEMENT OF ACTIVATOR BINDING TO CHROMATIN IN MAMMALIAN-CELLS
Autore:
BUNKER CA; KINGSTON RE;
Indirizzi:
MASSACHUSETTS GEN HOSP,DEPT MOL BIOL,WELLMAN 10 BOSTON MA 02114 MASSACHUSETTS GEN HOSP,DEPT MOL BIOL BOSTON MA 02114 HARVARD UNIV,SCH MED,DEPT GENET BOSTON MA 02115
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 20, volume: 93, anno: 1996,
pagine: 10820 - 10825
SICI:
0027-8424(1996)93:20<10820:ADEOAB>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA-POLYMERASE-II; TRANSCRIPTION FACTOR-BINDING; TUMOR VIRUS PROMOTER; FACTORS IN-VIVO; FACILITATED BINDING; NUCLEOSOMAL DNA; GENE-EXPRESSION; GAL4; COMPLEX; PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
C.A. Bunker e R.E. Kingston, "ACTIVATION DOMAIN-MEDIATED ENHANCEMENT OF ACTIVATOR BINDING TO CHROMATIN IN MAMMALIAN-CELLS", Proceedings of the National Academy of Sciences of the United Statesof America, 93(20), 1996, pp. 10820-10825

Abstract

DNA binding by transcriptional activators is typically an obligatory step in the activation of gene expression, Activator binding and subsequent steps in transcription are repressed by genomic chromatin. Studies in vitro have suggested that overcoming this repression is an important function of some activation domains. Here we provide quantitativein vivo evidence that the activation domain of GAL4-VP16 can increasethe affinity of GAL4 for its binding site on genomic DNA in mammaliancells. Moreover, the VP16 activation domain has a much greater stimulatory effect on expression from a genomic reporter gene than on a transiently transfected reporter gene, where factor binding is more permissive, We found that not all activation domains showed a greater activation potential in a genomic context, suggesting that only some activation domains can function in vivo to alleviate the repressive effects of chromatin, These data demonstrate the importance of activation domains in relieving chromatin-mediated repression in vivo and suggest thatone way they function is to increase binding of the activator itself.

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Documento generato il 29/03/20 alle ore 01:36:24