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Titolo:
INCREASED GLYCOGEN ACCUMULATION IN TRANSGENIC MICE OVEREXPRESSING GLYCOGEN-SYNTHASE IN SKELETAL-MUSCLE
Autore:
MANCHESTER J; SKURAT AV; ROACH P; HAUSCHKA SD; LAWRENCE JC;
Indirizzi:
WASHINGTON UNIV,SCH MED,DEPT MOL BIOL & PHARMACOL ST LOUIS MO 63110 UNIV INDIANAPOLIS,SCH MED,DEPT BIOCHEM & MOL BIOL INDIANAPOLIS IN 46227 UNIV WASHINGTON,SCH MED,DEPT BIOCHEM SEATTLE WA 98195 UNIV VIRGINIA,SCH MED,DEPT MED CHARLOTTESVILLE VA 22908 UNIV VIRGINIA,SCH MED,DEPT PHARMACOL CHARLOTTESVILLE VA 22908
Titolo Testata:
Proceedings of the National Academy of Sciences of the United Statesof America
fascicolo: 20, volume: 93, anno: 1996,
pagine: 10707 - 10711
SICI:
0027-8424(1996)93:20<10707:IGAITM>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUCOSE-TRANSPORT; PHOSPHORYLATION SITES; INSULIN; GLUT4; METABOLISM; ELEMENTS; FIBERS; 3A;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
J. Manchester et al., "INCREASED GLYCOGEN ACCUMULATION IN TRANSGENIC MICE OVEREXPRESSING GLYCOGEN-SYNTHASE IN SKELETAL-MUSCLE", Proceedings of the National Academy of Sciences of the United Statesof America, 93(20), 1996, pp. 10707-10711

Abstract

To investigate the role of glycogen synthase in controlling glycogen accumulation, we generated three lines of transgenic mice in which theenzyme was overexpressed in skeletal muscle by using promoter-enhancer elements derived from the mouse muscle creatine kinase gene, In all three lines, expression was highest in muscles composed primarily of fast-twitch fibers, such as the gastrocnemius and anterior tibialis. Inthese muscles, glycogen synthase activity was increased by as much as10-fold, with concomitant increases (up to 5-fold) in the glycogen content, The uridine diphosphoglucose concentrations were markedly decreased, consistent with the increase in glycogen synthase activity, Levels of glycogen phosphorylase in these muscles increased (up to 3-fold), whereas the amount of the insulin-sensitive glucose transporter 4 either remained unchanged or decreased, The observation that increasing glycogen synthase enhances glycogen accumulation supports the conclusion that the activation of glycogen synthase, as well as glucose transport, contributes to the accumulation of glycogen in response to insulin in skeletal muscle.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 14:48:36