Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ORGANIC OSMOLYTES IN AEROBIC-BACTERIA FROM MONO LAKE, AN ALKALINE, MODERATELY HYPERSALINE ENVIRONMENT
Autore:
CIULLA RA; DIAZ MR; TAYLOR BF; ROBERTS MF;
Indirizzi:
BOSTON COLL,MERKERT CHEM CTR,2609 BEACON ST CHESTNUT HILL MA 02167 BOSTON COLL,MERKERT CHEM CTR CHESTNUT HILL MA 02167 UNIV MIAMI,ROSENSTIEL SCH MARINE & ATMOSPHER SCI MIAMI FL 33149
Titolo Testata:
Applied and environmental microbiology
fascicolo: 1, volume: 63, anno: 1997,
pagine: 220 - 226
SICI:
0099-2240(1997)63:1<220:OOIAFM>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMPATIBLE SOLUTES; HALOPHILIC EUBACTERIA; METHANOBACTERIUM-THERMOAUTOTROPHICUM; ACCUMULATION; BIOSYNTHESIS; ALGAE; ACID; C-13;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
R.A. Ciulla et al., "ORGANIC OSMOLYTES IN AEROBIC-BACTERIA FROM MONO LAKE, AN ALKALINE, MODERATELY HYPERSALINE ENVIRONMENT", Applied and environmental microbiology, 63(1), 1997, pp. 220-226

Abstract

The identity and concentrations of intracellular organic solutes weredetermined by nuclear magnetic resonance spectroscopy for two strainsof aerobic, gram-negative bacteria isolated from Mono Lake, Calif, analkaline, moderately hypersaline lake, Ectoine (1,4,5,6-tetrahydro-2-methyl-4-pyrimidincarboxylic acid) was the major endogenous solute in both organisms, Concentrations of ectoine varied with external NaCl levels in strain ML-D but not in strain ML-G, where the level was high but invariant from 1.5 to 3.0 hi NaCl. Hydroxyectoine also occurred in strain ML-D, especially at elevated NaCl concentrations (2.5 and 3.0 M), but at levels lower than those of ectoine, Exogenous organic solutes that might occur in Mono Lake were examined for their effects on thede novo synthesis of ectoine, Dimethylsulfoniopropionate (DMSP) (0.1 or 1 mM) did not significantly lower ectoine levels in either isolate,and only strain R-IL-G showed any capacity for DMSP accumulation, With nitrogen limitation, however, DMSP (0.1 mM) substituted for ectoine in strain ML-G and became the main organic solute. Glycine betaine (GB) was more effective than DMSP in affecting ectoine levels, principally in strain ML-D. Strain ML-D accumulated GB to 50 or 67% of its organic solute pool at 2.5 M NaCl, at an external level of 0.1 or 1 mM GB, respectively, Strain ML-D also accumulated arsenobetaine, The methylated zwitterionic compounds, probably metabolic products of phytoplankton (DMSP and GB) or brine shrimps (arsenobetaine) in Mono Lake, may function as osmolytes for indigenous bacteria when present at high concentrations or under conditions of nitrogen limitation or salt stress.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 03:21:01