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Titolo:
PEROXYNITRITE-INDUCED APOPTOSIS IN T84 AND RAW-264.7 CELLS - ATTENUATION BY L-ASCORBIC-ACID
Autore:
SANDOVAL M; ZHANG XJ; LIU XP; MANNICK EE; CLARK DA; MILLER MJS;
Indirizzi:
LOUISIANA STATE UNIV,MED CTR,DEPT PEDIAT,SCH MED,1542 TULANE AVE NEW ORLEANS LA 70112 LOUISIANA STATE UNIV,MED CTR,DEPT PEDIAT,SCH MED NEW ORLEANS LA 70112
Titolo Testata:
Free radical biology & medicine
fascicolo: 3, volume: 22, anno: 1997,
pagine: 489 - 495
SICI:
0891-5849(1997)22:3<489:PAITAR>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE SYNTHASE; GUINEA-PIG ILEITIS; NF-KAPPA-B; SUPEROXIDE; OXIDATION; MACROPHAGES; ACTIVATION; EXPRESSION; THYMOCYTES; INJURY;
Keywords:
PEROXYNITRITE; APOPTOSIS; INFLAMMATION; ASCORBIC ACID; FREE RADICAL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
M. Sandoval et al., "PEROXYNITRITE-INDUCED APOPTOSIS IN T84 AND RAW-264.7 CELLS - ATTENUATION BY L-ASCORBIC-ACID", Free radical biology & medicine, 22(3), 1997, pp. 489-495

Abstract

The free radicals nitric oxide and superoxide react to form peroxynitrite (ONOO-), a potent cytotoxic oxidant. This study was designed to evaluate whether addition of L-Ascorbic acid (AsC) into the culture medium decreases peroxynitrite-induced apoptosis in human intestinal epithelial (T84) and murine macrophage (RAW 264.7) cell lines. In Experiment 1, T84 and RAW 264.7 cells were divided in two protocols: (1) treated with 100-300 mu M ONOO- and incubated for 4 h, and (2) treated with10-100 mu M ONOO- and incubated overnight (14 h). In Experiment 2, T84 and RAW 264.7 cells were treated with 300 mu M ONOO- and 500 mu M AsC and incubated for 4 h. In Experiment 3, T84 and RAW 264.7 cells werepreincubated for 2 h with 500 mu M AsC then exposed to 300 mu M ONOO-for 4 h. Cell viability (necrosis) was assessed by trypan blue dye exclusion. Apoptosis was quantified with a cell death detection ELISA assay. In the 4 h protocol, ONOO- induced apoptosis in T84 and RAW 264.7cells, at levels of 100-300 mu M. Concentrations of ONOO- greater than 300 mu M caused necrosis. In contrast, extension of the protocol to 14 h indicated that ONOO- induced apoptosis at lower concentrations (50; -75 mu M), with concentrations > 75 mu M resulting in necrosis. AsCadministered to the media or with preincubation plus washout, decreased peroxynitrite-induced apoptosis in T84 and RAW 264.7 cells. These results indicate that ONOO- may contribute to the pathophysiology of gut inflammation by promoting cell death and ascorbic acid may protect against peroxynitrite-induced damage. Copyright (C) 1996 Elsevier Science Inc.

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Documento generato il 02/12/20 alle ore 18:01:37