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Titolo:
A NOVEL TARGET FOR THE WILMS-TUMOR SUPPRESSOR PROTEIN (WT1) IS BOUND BY A UNIQUE COMBINATION OF ZINC FINGERS
Autore:
LITTLE MH; HOLMES G; PELL L; CARICASOLE A; DUARTE A; LAW M; WARD A; WAINWRIGHT B;
Indirizzi:
UNIV QUEENSLAND,CTR CELLULAR & MOL BIOL BRISBANE QLD 4072 AUSTRALIA UNIV QUEENSLAND,DEPT BIOCHEM BRISBANE QLD 4072 AUSTRALIA UNIV OXFORD,DEPT ZOOL,CRC,GROWTH FACTORS UNIT OXFORD OX1 3PS ENGLAND
Titolo Testata:
Oncogene
fascicolo: 7, volume: 13, anno: 1996,
pagine: 1461 - 1469
SICI:
0950-9232(1996)13:7<1461:ANTFTW>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
DENYS-DRASH SYNDROME; FACTOR-A-CHAIN; GENE-PRODUCT; DNA-BINDING; TRANSCRIPTION FACTOR; UROGENITAL DEVELOPMENT; PROMOTER ACTIVITY; POINT MUTATIONS; SEQUENCE; KIDNEY;
Keywords:
WILMS TUMOR SUPPRESSOR PROTEIN; WILMS TUMOR; TRANSCRIPTION FACTOR; ZINC FINGER BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
M.H. Little et al., "A NOVEL TARGET FOR THE WILMS-TUMOR SUPPRESSOR PROTEIN (WT1) IS BOUND BY A UNIQUE COMBINATION OF ZINC FINGERS", Oncogene, 13(7), 1996, pp. 1461-1469

Abstract

All isoforms of the Wilms' tumour suppressor protein, WT1, contain four consecutive zinc fingers which facilitate DNA binding. The predominant WT1 transcript contains a 9 base pair insertion resulting in an additional three amino acids, lysine-threonine-serine (KTS), between zinc fingers 3 and 4. WT1 zinc fingers 2, 3 and 4 are highly homologous to the zinc fingers of the early growth response gene, EGR1. However, only WT1-KTS is capable of binding an EGR1 consensus site. In contrast,the previously described genomic fragment, +P5 (D1S3309E), is bound by both WT1-KTS and WT1+KTS. In this study, the region within +P5 to which both WT1-KTS and WT1+KTS bind was defined as 5-GGAGAGGGAGGATC-3'. EGR1 did not bind +P5. By creating zinc finger deletions, we demonstrate that zinc finger 1, but not zinc finger 4, is critical for +P5 binding; whereas zinc finger 4, but not 1, is necessary for the binding ofWT1 target sites within EGR1, PDGF A chain and IGF2 promoters. Thus, zinc finger usage can vary with target and +P5 may represent a novel type of WT1 binding site, the physiological relevance of which must be investigated.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 02:49:05