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Titolo:
HUMAN CYCLIN B1 IS TARGETED TO THE NUCLEUS IN G(1) PHASE PRIOR TO ITSACCUMULATION IN THE CYTOPLASM
Autore:
DAVIDPFEUTY T; NOUVIANDOOGHE Y;
Indirizzi:
CTR UNIV ORSAY,INST CURIE,SECT RECH,BATIMENT 110 F-91405 ORSAY FRANCE
Titolo Testata:
Oncogene
fascicolo: 7, volume: 13, anno: 1996,
pagine: 1447 - 1460
SICI:
0950-9232(1996)13:7<1447:HCBITT>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
CDK-ACTIVATING KINASE; ROUS-SARCOMA VIRUS; HUMAN WEE1 KINASE; SEA-URCHIN EGGS; FISSION YEAST; CELL-CYCLE; SUBCELLULAR-LOCALIZATION; DNA-REPLICATION; PROTEIN-KINASE; S-PHASE;
Keywords:
CELL-CYCLE REGULATION; CYCLIN B1; CYCLIN A; TRANSFORMATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
56
Recensione:
Indirizzi per estratti:
Citazione:
T. Davidpfeuty e Y. Nouviandooghe, "HUMAN CYCLIN B1 IS TARGETED TO THE NUCLEUS IN G(1) PHASE PRIOR TO ITSACCUMULATION IN THE CYTOPLASM", Oncogene, 13(7), 1996, pp. 1447-1460

Abstract

The immunolocalisation patterns of cyclin B1 have been investigated in various tumour-derived and untransformed human cells, using one polyclonal (B7/B8) and tao different monoclonal anti-human cyclin B1 antibodies, GNS1 and GNS11. In actively dividing cell populations, GNS11 reveals uniquely a cytoplasmic pool of cyclin B1 that rapidly increases after the onset of S phase; yet, B7/B8 and GNS1 detect, besides this cytoplasmic cyclin B1 population, a moderate but clear nuclear concentration of the protein in cells that have not yet entered S phase. As for confluent populations of untransformed and tumour cells, they becomeenriched in G(1)- and G(2)-arrested cells that characteristically display a discreet nuclear or an intense cytoplasmic GNS1 immunostaining respectively. Altogether, our immunofluorescence data conjugated with the results of a detailed biochemical analysis suggest that human cyclin B1 would exist in situ as two distinguishable molecular entities differentially susceptible to in vitro degradation and exhibiting different timing, kinetics and site of accumulation during the cell cycle: one form (recognized by the polyclonal and GNS1 antibodies but apparently not by GNS11) starts to build up inside the nucleus prior to entry in S phase and the other (in which both the GNS11 and GNS1 epitopes are readily accessible) emerges and accumulates in the cytoplasm beyond the G(1)/S boundary.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/09/20 alle ore 18:34:30