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Titolo:
GTP-BINDING PROTEIN REGULATES THE CONTRACTILE RESPONSE ELICITED BY THE PHORBOL ESTER (PMA)-INDUCED ACTIVATION OF PROTEIN-KINASE-C IN THE ISOLATED RAT AORTA
Autore:
SUENAGA H; KASUYA Y; KAMATA K;
Indirizzi:
HOSHI UNIV,INST MED CHEM,DEPT PHYSIOL & MORPHOL,SHINAGAWA KU,EBARA 2-4-41 TOKYO 142 JAPAN HOSHI UNIV,INST MED CHEM,DEPT PHYSIOL & MORPHOL,SHINAGAWA KU TOKYO 142 JAPAN UPJOHN RES LABS,DEPT PHARMACOL TSUKUBA IBARAKI 30042 JAPAN
Titolo Testata:
General pharmacology
fascicolo: 6, volume: 27, anno: 1996,
pagine: 1035 - 1039
SICI:
0306-3623(1996)27:6<1035:GPRTCR>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
VASCULAR SMOOTH-MUSCLE; PERTUSSIS TOXIN; CALCIUM CHANNELS; CA-2+ CHANNELS; VASOCONSTRICTOR RESPONSES; INTRACELLULAR CALCIUM; ADENYLATE-CYCLASE; ADP-RIBOSYLATION; THORACIC AORTA; TAIL ARTERIES;
Keywords:
PHORBOL ESTER; PMA; GTP-BINDING PROTEIN; VOLTAGE-DEPENDENT CA2+-CHANNELS; ISLET-ACTIVATING PROTEIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
H. Suenaga et al., "GTP-BINDING PROTEIN REGULATES THE CONTRACTILE RESPONSE ELICITED BY THE PHORBOL ESTER (PMA)-INDUCED ACTIVATION OF PROTEIN-KINASE-C IN THE ISOLATED RAT AORTA", General pharmacology, 27(6), 1996, pp. 1035-1039

Abstract

1. The aim of the present study was to investigate the involvement ofGTP-binding protein in the contractile response induced by activationof protein kinase C (PKC) in isolated rat aorta. The rats were treated with islet activating protein (IAP) for 4 days prior to the experiments. 2. In the aorta from control rats, phorbol 12-myristate 13-acetate (PMA) produced biphasic contractions. The twitch contraction superimposed on the slowly developing contraction. The twitch contraction wasabolished by the removal of external Ca2+ or by treatment with nicardipine. In the aorta pretreated with IAP, PMA produced only a slowly developing contraction, and no twitch contraction was induced. 3. The application of Ca2+ to aortic strips in a Ca2+-free solution, that had been treated with 10(-6) M PMA caused concentration dependent contraction, and the contraction was completely inhibited by IAP. 4. Pretreatment with IAP inhibited Ca2+-induced contraction of the aorta in Ca2+-free medium in the presence of 10(-6) M clonidine, but did not affect the Ca2+-induced contraction in the medium treated with 10(-6) M phenylephrine and 10(-7) M nicardipine. 5. These results suggest that the activation of PKC by PMA produces biphasic contractions in the rat aorta. The twitch contraction may be induced by the activation of voltage-dependent Ca2+ channels. and the activation may be regulated by IAP-sensitive GTP-binding protein.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 18:56:46