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Titolo:
MICRODIALYSIS AND SPECT MEASUREMENTS OF AMPHETAMINE-INDUCED DOPAMINE RELEASE IN NONHUMAN-PRIMATES
Autore:
LARUELLE M; IYER RN; ALTIKRITI MS; ZEAPONCE Y; MALISON R; ZOGHBI SS; BALDWIN RM; KUNG HF; CHARNEY DS; HOFFER PB; INNIS RB; BRADBERRY CW;
Indirizzi:
NEW YORK STATE PSYCHIAT INST & HOSP,UNIT 28,177 W 168TH ST NEW YORK NY 10032 YALE UNIV,SCH MED,DEPT PSYCHIAT NEW HAVEN CT 06516 YALE UNIV,SCH MED,DEPT DIAGNOST RADIOL NEW HAVEN CT 06516 YALE UNIV,SCH MED,DEPT LAB MED NEW HAVEN CT 06516 VET ADM MED CTR W HAVEN CT 06516 UNIV PENN,DEPT RADIOL,DIV NUCL MED PHILADELPHIA PA 19104
Titolo Testata:
Synapse
fascicolo: 1, volume: 25, anno: 1997,
pagine: 1 - 14
SICI:
0887-4476(1997)25:1<1:MASMOA>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
POSITRON EMISSION TOMOGRAPHY; NAIVE SCHIZOPHRENIC-PATIENTS; RECEPTOR IMAGING AGENT; ENDOGENOUS DOPAMINE; HUMAN-BRAIN; INVIVO MICRODIALYSIS; KINETIC-PROPERTIES; H-3 RACLOPRIDE; D2 RECEPTORS; MOUSE-BRAIN;
Keywords:
MICRODIALYSIS; SPECT; AMPHETAMINE; ALPHA-METHYL-PARA-TYROSINE; DOPAMINE; DOPAMINE D-2 RECEPTORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
63
Recensione:
Indirizzi per estratti:
Citazione:
M. Laruelle et al., "MICRODIALYSIS AND SPECT MEASUREMENTS OF AMPHETAMINE-INDUCED DOPAMINE RELEASE IN NONHUMAN-PRIMATES", Synapse, 25(1), 1997, pp. 1-14

Abstract

The competition between endogenous transmitters and radiolabeled ligands for in vivo binding to neuroreceptors might provide a method to measure endogenous transmitter release in the living human brain with noninvasive techniques such as positron emission tomography (PET) or single photon emission computerized tomography (SPECT). In this study, wevalidated the measure of amphetamine-induced dopamine release with SPECT in nonhuman primates. Microdialysis experiments were conducted to establish the dose-response curve of amphetamine-induced dopamine release and to document how pretreatment with the dopamine depleter alpha-methyl-para-tyrosine (alpha MPT) affects this response. SPECT experiments were performed with two iodinated benzamides, [I-123]IBZM and [I-123]IBF, under sustained equilibrium condition. Both radiotracers are specific D-2 antagonists, but the affinity of [I-123]IBZM (K-D = 0.4 nM) is lower than that of [I-123]IBF (K-D = 0.1 nM). With both tracers, we observed a prolonged reduction in binding to D-2 receptors following amphetamine injection. [I-123]IBZM binding to D-2 receptors was moreaffected than [I-123]IBF by high doses of amphetamine, indicating that a lower affinity increases the vulnerability of a tracer to endogenous competition. With [I-123]IBZM, we observed an excellent correlationbetween reduction of D-2 receptor binding measured with SPECT and peak dopamine release measured with microdialysis after various doses of amphetamine. Pretreatment with alpha MPT significantly reduced the effect of amphetamine on [I-123]IBZM binding to D-2 receptors, confirmingthat this effect was mediated by intrasynaptic dopamine release. Together, these results validate the use of this SPECT paradigm as a noninvasive measurement of intrasynaptic dopamine release in the living brain. (C) 1997 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 16:11:33