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Titolo:
IN-VITRO COMPLEMENT-DEPENDENT BINDING AND IN-VIVO KINETICS OF PNEUMOCOCCAL POLYSACCHARIDE TI-2 ANTIGENS IN THE RAT SPLEEN MARGINAL ZONE ANDFOLLICLE
Autore:
HARMS G; HARDONK MJ; TIMENS W;
Indirizzi:
UNIV GRONINGEN,DEPT PATHOL,OOSTERSINGEL 63 NL-9713 EZ GRONINGEN NETHERLANDS
Titolo Testata:
Infection and immunity
fascicolo: 10, volume: 64, anno: 1996,
pagine: 4220 - 4225
SICI:
0019-9567(1996)64:10<4220:ICBAIK>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
INDEPENDENT TYPE-2 ANTIGENS; HUMAN LYMPHOCYTES-B; MONOCLONAL-ANTIBODIES; IMMUNE-RESPONSE; GERMINAL-CENTERS; MURINE SPLEEN; CELLS; MACROPHAGES; LOCALIZATION; METALLOPHILS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
G. Harms et al., "IN-VITRO COMPLEMENT-DEPENDENT BINDING AND IN-VIVO KINETICS OF PNEUMOCOCCAL POLYSACCHARIDE TI-2 ANTIGENS IN THE RAT SPLEEN MARGINAL ZONE ANDFOLLICLE", Infection and immunity, 64(10), 1996, pp. 4220-4225

Abstract

For a better understanding of the spleen-dependent induction of the humoral immune response against thymus-independent type 2 antigens, we have studied the in vitro and in vivo localization of different capsular pneumococcal polysaccharides (PPSs) in the rat spleen, In this study, we found that in vitro binding of PPS types 3, 4, 6B, 9N/V, 14, and23F was dependent on complement (probably a C3 fragment) and that thelocalization was predominantly restricted to the marginal-zone B lymphocytes and the follicular dendritic cells, In vivo, we observed with increase of time a shift of localized antigens, Shortly after injection, all PPS types localized in the marginal-zone B lymphocytes, then localized in the outer follicular mantle, and finally were found to be diffuse in the complete follicle and follicle corona, PPS types 3 and 9N/V and later also PPS type 23F localized additionally in red pulp macrophages. In particular, the localization in the marginal zone is important since the low flow in this area in combination with strongly CD21(+) B cells, which are activated early, gives a maximum opportunity for the induction of a primary humoral immune response with subsequent differentiation into plasma cells or migration to the germinal center,In addition, the localization of PPSs at follicular dendritic cells should be considered important in the induction of an ongoing immune response not restricted to the spleen.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 12:58:11