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Titolo:
ROLE OF PORPHYROMONAS-GINGIVALIS PROTEASE ACTIVITY IN COLONIZATION OFORAL-SURFACES
Autore:
TOKUDA M; DUNCAN M; CHO MI; KURAMITSU HK;
Indirizzi:
STATE UNIVERSITY NEW YORK BUFFALO,DEPT ORAL BIOL BUFFALO NY 14214 STATE UNIVERSITY NEW YORK BUFFALO,DEPT ORAL BIOL BUFFALO NY 14214 FORSYTH DENT CTR BOSTON MA 02115
Titolo Testata:
Infection and immunity
fascicolo: 10, volume: 64, anno: 1996,
pagine: 4067 - 4073
SICI:
0019-9567(1996)64:10<4067:ROPPAI>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRYPSIN-LIKE ACTIVITY; BACTEROIDES-GINGIVALIS; CYSTEINE PROTEINASE; PERIODONTAL-DISEASE; ARG-GINGIPAIN; STRUCTURAL CHARACTERIZATION; EPITHELIAL-CELLS; PURIFICATION; GENE; HEMAGGLUTININ;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
M. Tokuda et al., "ROLE OF PORPHYROMONAS-GINGIVALIS PROTEASE ACTIVITY IN COLONIZATION OFORAL-SURFACES", Infection and immunity, 64(10), 1996, pp. 4067-4073

Abstract

Cysteine proteases, including Arg-gingipain of Porphyromonas gingivalis, have been implicated as important virulence factors in periodontaldiseases, These enzymes are also involved in the hemagglutinating activity of the organisms, In order to determine the role of proteases inthe colonization of the gingival margin, we have compared the attachment properties of P. gingivalis 381 with those of its Arg-gingipain-defective mutant, G-102, Interactions with gram-positive bacteria, humanoral epithelial cells, extracellular matrix proteins, and type I collagen were evaluated, In all cases, mutant G-102 was deficient in attachment relative to the parental strain, The mutant's defects could be explained, in part, by the weak autoaggregation displayed by the mutant, which appeared to result from altered fimbrial expression, Both Western blot (immunoblot) and Northern (RNA) blot analyses indicated reduced expression of the major 43-kDa fimbrillin subunit in the mutant, These results suggest that Arg-gingipain may play both direct and indirect roles in the colonization of the gingival margin, In addition, fimbriae may play a direct role in interacting with some host surfaces.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 21:56:46