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Titolo:
FUNCTIONAL CORTICOTROPIN-RELEASING FACTOR RECEPTORS IN HUMAN NEUROBLASTOMA-CELLS
Autore:
DIETERICH KD; DESOUZA EB;
Indirizzi:
NEUROCRINE BIOSCI INC,3050 SCI PK RD SAN DIEGO CA 92037 NEUROCRINE BIOSCI INC SAN DIEGO CA 92037 UNIV CALIF SAN DIEGO,DEPT NEUROSCI SAN DIEGO CA 92037
Titolo Testata:
Brain research
fascicolo: 1, volume: 733, anno: 1996,
pagine: 113 - 118
SICI:
0006-8993(1996)733:1<113:FCFRIH>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTERIOR-PITUITARY CELLS; FACTOR CRF RECEPTORS; PROTEIN KINASE-C; ADENYLATE-CYCLASE ACTIVITY; CENTRAL NERVOUS-SYSTEM; AUTORADIOGRAPHIC LOCALIZATION; RAT-BRAIN; MOUSE PITUITARY; DOWN-REGULATION; TUMOR CELLS;
Keywords:
ADENYLATE CYCLASE; ACTH; CAMP; CANCER; PITUITARY GLAND; PROTEIN KINASE C; RECEPTOR DESENSITIZATION; RECEPTOR DOWN-REGULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
K.D. Dieterich e E.B. Desouza, "FUNCTIONAL CORTICOTROPIN-RELEASING FACTOR RECEPTORS IN HUMAN NEUROBLASTOMA-CELLS", Brain research, 733(1), 1996, pp. 113-118

Abstract

The present study examined the presence of functional corticotropin-releasing factor (CRF) receptors in IMR-32 neuroblastoma cells. [I-125]Tyr(o)-ovine CRF binding was linear with increasing protein concentrations, saturable, reversible and of high affinity. Scatchard analysis indicated a K-d of similar to 0.8 nM and a B-max of similar to 32 fmol/mg protein. Competition studies with CRF and related peptides revealeda pharmacological profile characteristic of the CRF(1) receptor subtype. CRF stimulated cAMP production in a dose-dependent manner with an apparent EC(50) of similar to 4 nM. In addition, the putative CRF receptor antagonist alpha-helical CRF(9-41) dose-dependently inhibited CRFstimulated (10 nM) cAMP production with an IC50 of similar to 60 nM. CRF treatment down regulated its own receptor while treatment with theprotein kinase C activator, phorbol 12-myristate 13-acetate (PMA), increased CRF binding in neuroblastoma cells. Taken together, these datademonstrate the utility of the human neuroblastoma cell line for functional studies on CRF receptors and suggest that CRF may play a regulatory role in the pathophysiology of human neuroblastoma.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 22:20:56