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Titolo:
HIGH-SENSITIVITY FLUORESCENCE DERIVATIZATION FOR THE DETERMINATION OFHYDROXY COMPOUNDS IN AQUEOUS-SOLUTION BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
Autore:
MOTTE JC; WINDEY R; DELAFORTRIE A;
Indirizzi:
MINIST AGR,INST RECH CHIM,LEUVENSESTEENWEG 17 B-3080 TERVUREN BELGIUM WATERS BELGIUM SA B-1130 BRUSSELS BELGIUM
Titolo Testata:
Journal of chromatography
fascicolo: 1-2, volume: 728, anno: 1996,
pagine: 333 - 341
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED CARBAMATE REAGENT; PRE-COLUMN DERIVATIZATION; FATTY-ACIDS;
Keywords:
DERIVATIZATION, LC; BEVERAGES; 6-SMINOQUINOLYL-N-HYDROXYSUCCINIMIDYL CARBAMATE; ALCOHOLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
J.C. Motte et al., "HIGH-SENSITIVITY FLUORESCENCE DERIVATIZATION FOR THE DETERMINATION OFHYDROXY COMPOUNDS IN AQUEOUS-SOLUTION BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY", Journal of chromatography, 728(1-2), 1996, pp. 333-341

Abstract

The utility of the fluorescent labelling reagent 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) for the determination of alcohols inaqueous media was evaluated. The labelling conditions were optimized for C-1-C-8 alcohols. Under the very mild reaction conditions of 25 degrees C for 1 min in a basic buffer, all the alcohols tested were derivatized with the reagent to yield highly stable fluorescent carbamate derivatives. The maximum excitation (290 nm) and emission (365 nm) wavelengths were the same for all the alcohols tested. The stability of the derivatives and their yield were affected by the chosen pH in the range 6-9.5 and by the steric hindrance of the alcohol function. All the derivatives obtained with AQC were completely separated by reversed-phase high-performance liquid chromatography on a 20-cm C-18 column with a gradient of aqueous sodium acetate buffer (pH 6) and acetonitrile. The detection limit (S/N = 3) with fluorescence detection is at the picomole level. Some preliminary applications in the fields of foods and beverages are described.

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Documento generato il 09/04/20 alle ore 19:59:34