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Titolo:
NUCLEAR AND CELLULAR MORPHOMETRY OF NORMAL AND POTENTIALLY PREMALIGNANT EPITHELIUM DURING EPITHELIAL CELLULAR-DIFFERENTIATION AND MATURATION IN HUMAN LARGE-BOWEL CRYPTS
Autore:
TIPOE GL; WHITE FH;
Indirizzi:
UNIV HONG KONG,FAC MED,DEPT ANAT,LI SHU FAN BLDG,5 SASSON RD HONG KONG HONG KONG
Titolo Testata:
GI cancer
fascicolo: 1, volume: 2, anno: 1996,
pagine: 13 - 21
SICI:
1064-9700(1996)2:1<13:NACMON>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSITIONAL MUCOSA; COLON; CARCINOMAS; INTESTINE; RENEWAL; CANCER;
Keywords:
HUMAN; COLORECTUM; MORPHOMETRY; DIFFERENTIATION; PRECANCERS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
G.L. Tipoe e F.H. White, "NUCLEAR AND CELLULAR MORPHOMETRY OF NORMAL AND POTENTIALLY PREMALIGNANT EPITHELIUM DURING EPITHELIAL CELLULAR-DIFFERENTIATION AND MATURATION IN HUMAN LARGE-BOWEL CRYPTS", GI cancer, 2(1), 1996, pp. 13-21

Abstract

This study investigates whether variations of nuclei and cells exist during differentiation of normal (N) and potentially premalignant (PPM) epithelia of the human large bowel using morphometric techniques. Samples were obtained from resected large bowels with malignant lesions of 10 Chinese patients. N samples were biopsied from the margins of each resected large bowel. PPM samples were obtained within 2 cm of the margins of the malignant lesions. Tissues were processed for routine histology under strictly standardised schedules and 4 mu m serial sections were stained with H&E. Crypts were divided into basal(B), intermediate (I) and surface (S) segments using strict criteria. Interactive digitisation was performed using a Zeiss VIDAS image analyser and morphometric parameters quantified comprised nucleo-cytoplasmic (N/C) ratioand nuclear (V-N), cytoplasmic (V-CYT) and cellular (V-CELL) volumes,major (a) and minor (b) axes, nuclear axial (a/b) ratio, nuclear area(AN), nuclear perimeter (PERIM) and nuclear diameter (D). The largestnuclei (A(N), D, V-N) were observed in S segments and differed significantly from the nuclei of the other two segments of N and PPM crypts. Nuclear size of PPM crypts in all segments was significantly smaller than the nuclei of corresponding segments of N crypts. Data for PERIM and a/b ratio suggested that the nuclei at the surface segment of N and PPM crypts were less circular in shape and had longer boundaries than nuclei from other segments. Nuclear volumetric variations and the shift of nuclear profile shape from circular to ellipsiodal between B and S segments might reflect an increase in functional and metabolic requirements of the actively dividing and differentiating cells at the B and I segments of the N and PPM crypts. The patterns of change in N crypts were similarly expressed in PPM crypts and distinct differences existed in crypts between these two groups when comparing similar segments. All values in PPM were consistently lower when compared with N crypts. These initial observations may represent a subtle altered state of cellular differentiation in PPM which may be a reflection of early preneoplastic transformation.

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Documento generato il 09/07/20 alle ore 14:01:17