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Titolo:
NUCLEAR AND CYTOPLASMIC DYNAMICS OF SPERM PENETRATION, PRONUCLEAR FORMATION AND MICROTUBULE ORGANIZATION DURING FERTILIZATION AND EARLY PREIMPLANTATION DEVELOPMENT IN THE HUMAN
Autore:
VANBLERKOM J; DAVIS P; MERRIAM J; SINCLAIR J;
Indirizzi:
UNIV COLORADO,DEPT MOLEC CELLULAR & DEV BIOL BOULDER CO 80309 REPROD GENET IN VITRO DENVER CO 80222
Titolo Testata:
Human reproduction update
fascicolo: 5, volume: 1, anno: 1995,
pagine: 429 - 461
SICI:
1355-4786(1995)1:5<429:NACDOS>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
MATURE HUMAN OOCYTES; GAMMA-TUBULIN; INVITRO FERTILIZATION; MOUSE OOCYTES; CELL-CYCLE; REORGANIZATION; EMBRYOS; MEIOSIS; EGGS;
Keywords:
CENTROSOME; CYTOSKELETAL ORGANIZATION; MITOTIC SPINDLES; PENETRATION; PRONUCLEAR EVOLUTION;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
47
Recensione:
Indirizzi per estratti:
Citazione:
J. Vanblerkom et al., "NUCLEAR AND CYTOPLASMIC DYNAMICS OF SPERM PENETRATION, PRONUCLEAR FORMATION AND MICROTUBULE ORGANIZATION DURING FERTILIZATION AND EARLY PREIMPLANTATION DEVELOPMENT IN THE HUMAN", Human reproduction update, 1(5), 1995, pp. 429-461

Abstract

This report describes spatial and temporal aspects of sperm penetration and intracytoplasmic migration, pronuclear evolution and the specificity of presyngamic opposition, stage-specific changes in cytoskeletal organization and the relative contribution of maternal and paternal components to mitotic spindle formation, These studies involved observations of living human oocytes during conventional insemination in vitro and after intracytoplasmic deposition of spermatozoa, analysis of chromatin organization and distribution during pronuclear evolution, and detection of actin and alpha-, beta- and gamma-tubulin by confocal immuno-fluorescence microscopy, Immature and mature oocytes, penetratedbut unfertilized oocytes, fertilized but arrested eggs, and cleavage-stage embryos from normal and dispermic fertilizations were examined, The results demonstrate that sperm nuclear migration to the maternal perinuclear region is rapid and linear, occurs in the absence of a detectable cytoskeletal system and appears to be assisted by an unusual configuration of the sperm tail principal piece which results from either retained intracytoplasmic motility or the process by which the spermtail is progressively incorporated into the oocyte, Our findings alsoshow a specificity of pronuclear alignment that is associated with a polarized distribution of both maternal and paternal chromatin, and with the position of the sperm centrosome and the presence of microtubules nucleated from this structure, The results also indicate that a maternal microtubule nucleating capacity is present in the immature oocyte but is apparently inactive until spindle formation, The poles of thefirst mitotic spindle appear to be derived from the sperm centrosome,although some maternal contribution cannot be excluded, The sperm tail and centrosome persist in a single cell through the cleavage stages,and the latter serves as a prominent site of cytoplasmic microtubule nucleation, The results provide a detailed understanding of the cellular and nuclear morphodynamics of the human fertilization process and indicate subtle defects that may be responsible for early developmentalfailure.

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Documento generato il 23/09/20 alle ore 14:55:02