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Titolo:
RADIOTRACER BINDING TO BRAIN MICROSOMES DETERMINED BY THIN-LAYER CHROMATOGRAPHY
Autore:
ZAMORA PO; STRATESTEFFAN M; GUHLKE S; SASS KS; CARDILLO A; BENDER H; BIERSACK HJ;
Indirizzi:
RHO MED INC,4261 BALLOON PK,RD NE ALBUQUERQUE NM 87109 UNIV BONN,NUKL MED KLIN W-5300 BONN GERMANY
Titolo Testata:
Nuclear medicine and biology
fascicolo: 1, volume: 23, anno: 1996,
pagine: 61 - 67
SICI:
0969-8051(1996)23:1<61:RBTBMD>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALPHA-METHYL TYROSINE; L-3-)I-123>IODO-ALPHA-METHYL TYROSINE; INVIVO APPLICATION; SOMATOSTATIN; TUMORS; SPECT; ANALOGS; BLOOD;
Keywords:
THIN LAYER CHROMATOGRAPHY (TLC); RADIOTRACER BINDING; MICROSOMES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
P.O. Zamora et al., "RADIOTRACER BINDING TO BRAIN MICROSOMES DETERMINED BY THIN-LAYER CHROMATOGRAPHY", Nuclear medicine and biology, 23(1), 1996, pp. 61-67

Abstract

A thin-layer chromatography (TLC) assay was developed to monitor the interaction of radiotracers with brain microsomes. Murine brain microsomes were coated onto a zone of a TLC strip, the unreacted sites blocked with gelatin, and the radiotracers chromatographed over the microsomes. Radiotracers bound to the microsomes and were separated from the unreacted materials which migrated at or near the solvent front. Up to80% of the applied radioactivity bound to the brain microsomes when using Tc-99m-(d,l) hexamethyl-propyleneamine oxime (HMPAO) and thoxy-N-[(1-ethyl-2-pyrrolidinyl)methyl]-benzamide (I-123-IBZM) as tracers. Onthe other hand, the presumptive negative control materials p-I-15-phenyl-pentadecanoic acid-I-123 (I-123-IPPA) and Tc-99m-mercapto-acetyl triglycine (MAG3) bound poorly (7% and 4%, respectively). Tc-99m-ethyl cysteinate dimer (ECD) interacted poorly (9.9%), a result thought to be consistent with its known inability to be metabolized by nonprimate brain tissue. Radiolabeled octreotide analogues (radiolabeled with In-111, I-131 or Tc-99m) also bound, and the binding could be reduced by excess unlabeled octreotide. Also, chemical modification by acylation of Lys(5) in In-111-labeled octreotide led to decreased binding (approximately 70%) compared to the original radiotracer. Chromatography of the various radiotracers over TLC strips coated only with gelatin was used to monitor nonspecific binding and was low and frequently below 5%. This technique does not require wash steps or centrifugation, and assays are rapidly completed. The assay could be useful in monitoring the interaction of radiotracers with brain microsomes and in evaluatingand developing new radiotracers.

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Documento generato il 21/01/21 alle ore 07:13:40