Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
EXTENSIVE AMPLIFICATION OF SINGLE CELLS FROM CD34(-CORD BLOOD AND IDENTIFICATION OF LONG-TERM CULTURE-INITIATING CELLS PRESENT IN 2 SUBSETS() SUBPOPULATIONS IN UMBILICAL)
Autore:
DEWYNTER EA; NADALI G; COUTINHO LH; TESTA NG;
Indirizzi:
PATERSON INST CANC RES,DEPT EXPTL HAEMATOL,CRC MANCHESTER M20 9BX LANCS ENGLAND
Titolo Testata:
Stem cells
fascicolo: 5, volume: 14, anno: 1996,
pagine: 566 - 576
SICI:
1066-5099(1996)14:5<566:EAOSCF>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC PROGENITOR CELLS; BONE-MARROW CELLS; MYELOID PROGENITORS; PERIPHERAL-BLOOD; STEM-CELLS; IN-VITRO; EXPANSION; DIFFERENTIATION; INVITRO; TRANSPLANTATION;
Keywords:
CD34(+) SUBSETS; AMPLIFICATION; LONG-TERM CULTURE-INITIATING CELLS; CORD BLOOD; COLONY-FORMING CELLS; PRIMITIVE CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
E.A. Dewynter et al., "EXTENSIVE AMPLIFICATION OF SINGLE CELLS FROM CD34(-CORD BLOOD AND IDENTIFICATION OF LONG-TERM CULTURE-INITIATING CELLS PRESENT IN 2 SUBSETS() SUBPOPULATIONS IN UMBILICAL)", Stem cells, 14(5), 1996, pp. 566-576

Abstract

CD34(+) cord blood cells were isolated with immunomagnetic beads and fractionated by fluorescence-activated cell sorting (FAGS) into three subpopulations: CD34(+)38(+)DR(+), CD34(+)38(-)DR(+) and CD34(+)38(-)DR(-), using antibodies specific for these cell surface markers, Cells from each of the three subsets were plated as single cells in serum-free medium supplemented with a combination of growth factor and individual cells were monitored for proliferation and the capacity to form colony-forming cells, Single cells from the CD34(+)38(+)DR(+) subset showed the lowest expansion capacity, generating up to 1.1 x 10(6) cells at five weeks, while individual cells from both the CD34(+)38(-)DR(+) and CD34(+)38(-)DR(-) subsets could be expanded up to 1.8 x 10(6) and 9.2 x 10(6) cells, respectively, over a period of six weeks, The different subpopulations also generated colony-forming cells which gave rise to erythroid, myeloid and erythroid/myeloid colonies, CD34(+)38(-)DR(+) cells generated large numbers of colonies within two weeks in liquid culture, but this rapidly declined, Generation of lineage-committedcolony-forming cells was better sustained in the CD34(+)38(-)DR(-) population and continued for up to six weeks in culture. Overall, the generation of colony-forming cells declined with time in culture, although the cell numbers continued to expand, How;el er, when the same populations were plated on irradiated bone marrow stroma, both the CD34(+)38(-)DR(+) and the CD34(+)38(-)DR(-) cells were capable of producing granulocyte-macrophage colony-forming cells (GM-CFCs) for 10 to 12 weeks, As hemopoiesis was sustained for almost three months, it appears that these populations were significantly enriched in long-term culture-initiating cells (LTC-ICs), Although both populations generated GM-CFCs, the CD34(+)38(-)DR(-) cells sustained production of higher numbers of colony-forming cells than the CD34(+)38(-)DR(-) population, These results demonstrate that cells from cord blood can be efficiently monitored at the single-cell level for proliferation, expansion and colony-forming capacity, Furthermore, at least two populations of LTC-ICs canbe distinguished in cord blood CD34(+)38(-) cells by the differentialexpression of the KLA-DR antigen.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 10:19:04