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Titolo:
HUMAN DELAYED-TYPE HYPERSENSITIVITY REACTION IN A SCID MOUSE ENGRAFTED WITH HUMAN T-CELLS AND AUTOLOGOUS SKIN
Autore:
PETZELBAUER P; GROGER M; KUNSTFELD R; PETZELBAUER E; WOLFF K;
Indirizzi:
UNIV VIENNA,SCH MED,DEPT DERMATOL,DIV GEN DERMATOL,WAEHRINGER GUERTEL18-20 A-1090 VIENNA AUSTRIA
Titolo Testata:
Journal of investigative dermatology
fascicolo: 4, volume: 107, anno: 1996,
pagine: 576 - 581
SICI:
0022-202X(1996)107:4<576:HDHRIA>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN PERIPHERAL-BLOOD; COMBINED IMMUNODEFICIENT MOUSE; MICE; LYMPHOCYTES; CHIMERAS; MODEL; MOLECULES; CULTURE; SYSTEM;
Keywords:
TETANUS; ENDOTHELIUM; VACCINATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
P. Petzelbauer et al., "HUMAN DELAYED-TYPE HYPERSENSITIVITY REACTION IN A SCID MOUSE ENGRAFTED WITH HUMAN T-CELLS AND AUTOLOGOUS SKIN", Journal of investigative dermatology, 107(4), 1996, pp. 576-581

Abstract

We have developed an animal model to study human delayed-type hypersensitivity reactions occurring in a human environment within a mouse host, Human skin was grafted onto the backs and autologous human immune cells were injected into the peritoneal cavity of mice with severe combined immunodeficiency. Seven and 14 d after grafting, 2-50% of total white blood and spleen cells were of human origin. Mouse spleen-derived human T cells from tetanus toxoid-sensitized donors proliferated in response to tetanus toroid as measured by [H-3]thymidine uptake, and the strength of this proliferative response equaled that with pre-graftT cells from the same donor. Proliferation was blocked with monoclonal antibodies to human but not to mouse major histocompatibility complex antigens and with anti-human CD4 monoclonal antibodies. In vivo vaccination of mice with tetanus toroid did not enhance proliferation of mouse spleen-derived human T cells in response to antigen. Injection oftetanus toroid into the human skin graft caused a perivascular human CD4(+)/CD25(+) T-cell infiltrate, which was not present when tetanus toroid was injected into adjacent mouse skin. We conclude that human T cells grafted into mice with severe combined immunodeficiency retain their function, that human T cells specifically recognize human but notmouse skin as homing sites, and that human T-cell responses depend onthe human microenvironment. This model lends itself to studies of endothelium-T-cell interactions, T-cell activation within skin, and chronic inflammatory skin diseases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 07:40:45