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Titolo:
DEVELOPMENT OF PORCINE BLASTOCYSTS FROM IN-VITRO-MATURED AND ACTIVATED HAPLOID AND DIPLOID OOCYTES
Autore:
KUREBAYASHI S; MIYAKE M; KATAYAMA M; MIYANO T; KATO S;
Indirizzi:
KOBE UNIV,FAC AGR,LAB ANIM REPROD,NAKA KU KOBE 657 JAPAN KOBE UNIV,FAC AGR,GRAD SCH SCI & TECHNOL KOBE 657 JAPAN
Titolo Testata:
Theriogenology
fascicolo: 6, volume: 46, anno: 1996,
pagine: 1027 - 1036
SICI:
0093-691X(1996)46:6<1027:DOPBFI>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
PARTHENOGENETIC DEVELOPMENT; BOVINE OOCYTES; IN-VITRO; NUCLEAR TRANSPLANTATION; CHEMICAL ACTIVATION; MATURATION INVITRO; MOUSE OOCYTES; EMBRYOS; STIMULATION; ETHANOL;
Keywords:
PORCINE OOCYTES; MATURATION IN VITRO; PLOIDY; PARTHENOGENESIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
S. Kurebayashi et al., "DEVELOPMENT OF PORCINE BLASTOCYSTS FROM IN-VITRO-MATURED AND ACTIVATED HAPLOID AND DIPLOID OOCYTES", Theriogenology, 46(6), 1996, pp. 1027-1036

Abstract

The purpose of this study was to determine the developmental capacityof electro-activated porcine oocytes. Follicular oocytes collected from gilts at local slaughterhouses were matured for 48 h and were then subjected to a single square pulse of direct current for 100 mu sec at1,500 V/cm for activation. To obtain activated diploid oocytes, some were treated with 5.0 mu g/ml cytochalasin B for 4 h immediately afterelectro-activation. The frequency of activation ranged from 96 to 100%. While 91% of activated oocytes that had not been treated with cytochalasin B had 2 polar bodies and a nucleus (haploids), 92% of the oocytes treated with cytochalasin B had only the first polar body and 2 nuclei (diploids). Haploids and diploids were further cultured in TCM-199 medium that contained 10% (v/v) heat-treated fetal calf serum (FCS) and 0.1 mg/ml sodium pyruvate (mTCM) or in Whitten's medium plus 0.4% (w/v) bovine serum albumin (BSA). The frequency of abnormal oocytes was significantly higher in mTCM (83%) than in Whitten's medium (65%) 96h after the electroactivation (p<0.01), suggesting that Whitten's medium supported the development of activated oocytes beyond the morula stage. In all cases, several oocytes developed to the blastocyst stage 144 h after electro-activation (1 to 12%). The frequency was significantly higher in the case of diploids cultured in Whitten's medium (12%)(P<0.01) than in the case of haploids cultured in Whitten's medium (4%), or in the case of haploids cultured in mTCM (1%). The mean number of nuclei per blastocyst was significantly lower in mTCM (haploids, 15; diploids, 16.1) than in Whitten's medium (haploids, 35.7; diploids, 40.1; P<0.01), suggesting that the number of nuclei in blastocysts wasaffected by the culture medium.

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Documento generato il 22/10/20 alle ore 03:32:01