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Titolo:
LIGHT-MICROSCOPIC AND ULTRASTRUCTURAL-CHANGES IN THE CECA OF CHICKS INOCULATED WITH HUMAN AND CANINE SERPULINA-PILOSICOLI
Autore:
MUNIAPPA N; DUHAMEL GE; MATHIESEN MR; BARGAR TW;
Indirizzi:
UNIV NEBRASKA,DEPT VET & BIOMED SCI,VET BASIC SCI BLDG,ROOM 147 LINCOLN NE 68583
Titolo Testata:
Veterinary pathology
fascicolo: 5, volume: 33, anno: 1996,
pagine: 542 - 550
SICI:
0300-9858(1996)33:5<542:LAUITC>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
TREPONEMA-HYODYSENTERIAE; ESCHERICHIA-COLI; INTESTINAL SPIROCHETOSIS; EXPERIMENTAL-INFECTION; HELICOBACTER-PYLORI; CYTOKINE PRODUCTION; COLONIC EPITHELIUM; INNOCENS; MUCOSA; CELLS;
Keywords:
BACTERIAL ADHESION; BACTERIAL ATTACHMENT; BETA-HEMOLYTIC INTESTINAL SPIROCHETES; CECAL EPITHELIUM CHICKS; INTESTINAL SPIROCHETOSIS; MICROVILLOUS EFFACEMENT; SERPULINA PILOSICOLI;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
N. Muniappa et al., "LIGHT-MICROSCOPIC AND ULTRASTRUCTURAL-CHANGES IN THE CECA OF CHICKS INOCULATED WITH HUMAN AND CANINE SERPULINA-PILOSICOLI", Veterinary pathology, 33(5), 1996, pp. 542-550

Abstract

Light microscopic and ultrastructural changes were observed in chickschallenged with North American Serpulina pilosicoli, a weakly beta-hemolytic intestinal spirochete (WBHIS) associated with human and canineintestinal spirochetosis. Chicks in control groups received trypticase soy broth or canine Serpulina innocens. The birds were necropsied atweekly intervals, and the ceca were processed for bacteriologic and pathologic examinations. No WBHIS were isolated from the ceca of chicksin the control groups, but WBHIS with genotypes similar to the parentisolates were isolated from the ceca of chicks inoculated with human and canine S. pilosicoli. Gross examination revealed no significant changes in the ceca of chicks at any time postinoculation. Light microscopic examination revealed no spirochetal attachment in the ceca of chicks in control groups. In contrast, focal to diffuse thickening of thebrush border of the surface epithelium along with dilation of the crypt lumina and mild focal lamina propria heterophil infiltration were present in the ceca of chicks inoculated with human and canine S. pilosicoli. Scanning electron microscopic examination revealed focal to confluent spirochetal attachment mainly in the furrow region at the periphery of the crypt units. Transmission electron microscopic examinationrevealed spirochetes attached to the brush border of the cecal epithelium, causing effacement of the microvilli and disruption of the terminal web microfilaments. The cecal epithelium of chicks inoculated withthe canine S. pilosicoli also had caplike elevations of the apical membrane at the point of attachment of the spirochetes together with large numbers of vesicles in the cytoplasm immediately beneath the terminal web and evidence of spirochetal invasion beyond the mucosal barrier. The changes observed suggested that the mechanism of attachment of human and canine S. pilosicoli to the cecal epithelium of chicks was analogous to but different from that described previously for other attaching and effacing gastroenteric bacterial pathogens of human beings and animals.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 12:29:30