Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
IMMUNE-RESPONSES TO LINEAR EPITOPES ON THE PORB PROTEIN OF NEISSERIA-MENINGITIDIS IN PATIENTS WITH SYSTEMIC MENINGOCOCCAL DISEASE
Autore:
DELVIG AA; WEDEGE E; MICHAELSEN TE; HOIBY EA; BRANDTZAEG P; ROSENQVIST E;
Indirizzi:
NATL INST PUBL HLTH,DEPT VACCINOL N-0403 OSLO NORWAY NATL INST PUBL HLTH,DEPT BACTERIOL N-0403 OSLO NORWAY ULLEVAL UNIV HOSP,DEPT INFECT DIS N-0407 OSLO NORWAY
Titolo Testata:
Microbiology
, volume: 142, anno: 1996,
parte:, 9
pagine: 2491 - 2498
SICI:
1350-0872(1996)142:<2491:ITLEOT>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
OUTER-MEMBRANE PROTEIN; T-CELL EPITOPES; SEROGROUP-A; ASYMPTOMATIC CARRIAGE; MONOCLONAL-ANTIBODIES; COMPLEMENT-DEFICIENT; BACTERIAL-MENINGITIS; PEPTIDE-SYNTHESIS; GONOCOCCAL PORIN; VESICLE VACCINE;
Keywords:
NEISSERIA MENINGITIDIS; SYSTEMIC MENINGOCOCCAL DISEASE; PORB PROTEIN; B-CELL EPITOPE; SYNTHETIC PEPTIDES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
58
Recensione:
Indirizzi per estratti:
Citazione:
A.A. Delvig et al., "IMMUNE-RESPONSES TO LINEAR EPITOPES ON THE PORB PROTEIN OF NEISSERIA-MENINGITIDIS IN PATIENTS WITH SYSTEMIC MENINGOCOCCAL DISEASE", Microbiology, 142, 1996, pp. 2491-2498

Abstract

Neisserial porins, the major protein constituents of the outer membrane capable of inducing antibody responses in humans, are considered tobe meningococcal vaccine candidates, so it is important to map the relevant B-cell epitopes. For B-cell epitope analyses of the serotype 15PorB protein in Neisseria meningitidis, paired sera from selected patients with systemic meningococcal disease (SMD) were screened with synthetic 12mer peptides spanning the PorB protein sequence, and/or its variable region 1 (VR1). A 'SMD-related' linear B-cell epitope was found within the VR1 region consisting of 14 residues ((17)svFHQNGQVTEvtt(30)). A 23mer soluble peptide (D63b2) that covered the VR1 region, including the complete (17)svFHQNGQVTEvtt(30) sequence, was recognized, whereas no detectable binding was observed to a 16mer peptide (D63a1) containing most of the essential sequence ((19)FHQNGQVTEVtt(30)). A lowfrequency of IgG responses specific for the PorB linear epitopes was found in convalescent-phase sera from 132 SMD patients studied, as judged from both immunoblotting studies (24/132; 18.2 %) and reactivity with peptide D63b2 (18/132; 13.6 %). Peptide D63b2 significantly inhibited IgG binding to the denatured PorB protein on immunoblots, suggesting that this B-cell epitope was one of the main linear epitopes on thePorB protein recognized by sera from some SMD patients.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 18:34:39