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Titolo:
GENE ACTIVATION BY RECRUITMENT OF THE RNA-POLYMERASE-II HOLOENZYME
Autore:
FARRELL S; SIMKOVICH N; WU YB; BARBERIS A; PTASHNE M;
Indirizzi:
HARVARD UNIV,DEPT MOL & CELLULAR BIOL CAMBRIDGE MA 02138 HARVARD UNIV,DEPT MOL & CELLULAR BIOL CAMBRIDGE MA 02138 UNIV ZURICH,INST MOL BIOL ZURICH SWITZERLAND
Titolo Testata:
Genes & development
fascicolo: 18, volume: 10, anno: 1996,
pagine: 2359 - 2367
SICI:
0890-9369(1996)10:18<2359:GABROT>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
SICKLE-CELL-ANEMIA; EUKARYOTIC TRANSCRIPTIONAL ACTIVATORS; BINDING PROTEIN; YEAST; BASES; GAL4; DNA; TBP; COOPERATE; PROMOTER;
Keywords:
RNA POLYMERASE II HOLOENZYME; GAL4; GAL11; GENE ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
S. Farrell et al., "GENE ACTIVATION BY RECRUITMENT OF THE RNA-POLYMERASE-II HOLOENZYME", Genes & development, 10(18), 1996, pp. 2359-2367

Abstract

The single amino acid ''P'' (potentiator) mutation in the holoenzyme component GAL11 creates an interaction between that protein and the dimerization region of GAL4. That interaction triggers strong gene activation when the GAL4 fragment is tethered to DNA. Here we show that, among a series of variants of the GAL4 dimerization region and differentGAL11P alleles, the strength of the interaction as quantitated in vitro correlates with the degree of activation in vivo; swapping the protein fragments bearing the GAL4 dimerization region and the GAL11P mutation such that the latter is tethered to DNA and the former is attached to the holoenzyme does not diminish gene activation; gene activationin this system is squelched by overproduction of either a fragment bearing the GAL4 dimerization region or a fragment of GAL11 bearing a P mutation; and neither GAL11 nor GAL11P is a target of an acidic activating region. These results argue that the GAL4-GAL11P interaction triggers gene activation simply by recruiting the holoenzyme to DNA. Consistent with this view, we also show that fusion of LexA to another holoenzyme component, SRB2, creates an activator, and that an SRB2 mutant predicted on genetic grounds to interact especially efficiently with aholoenzyme containing a specific mutant form of polymerase also activates more efficiently when tethered to DNA.

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Documento generato il 22/10/20 alle ore 03:17:17