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Titolo:
ASSIGNMENT OF PROTEIN DISULFIDES BY A COMPUTER METHOD USING MASS-SPECTROMETRIC DATA
Autore:
CAPORALE C; SEPE C; CARUSO C; PUCCI P; BUONOCORE V;
Indirizzi:
UNIV TUSCIA,DIPARTIMENTO AGROBIOL & AGROCHIM,VIA S CAMILLO DE LELLIS I-01100 VITERBO ITALY UNIV NAPLES FEDERICO II,DIPARTIMENTO CHIM ORGAN & BIOL NAPLES ITALY CNR,SERV SPETTROMETRIA MASSA I-80125 NAPLES ITALY
Titolo Testata:
FEBS letters
fascicolo: 2-3, volume: 393, anno: 1996,
pagine: 241 - 247
SICI:
0014-5793(1996)393:2-3<241:AOPDBA>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALPHA-AMYLASE INHIBITOR; AMINO-ACID-SEQUENCE; DISULFIDE BONDS; BRIDGES; PEPTIDES; INSULIN;
Keywords:
DISULFIDE BRIDGE; PROTEIN STRUCTURE; COMPUTER METHOD; MASS SPECTROMETRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
C. Caporale et al., "ASSIGNMENT OF PROTEIN DISULFIDES BY A COMPUTER METHOD USING MASS-SPECTROMETRIC DATA", FEBS letters, 393(2-3), 1996, pp. 241-247

Abstract

We designed a computer program for the assignment of protein disulphides using mass spectrometric data. All the theoretical linear peptidescontaining from one to three cysteines are generated on the basis of the protein sequence. Their combination ways are determined in order to create all the possible disulphide-bridged fragments containing fromtwo to six cysteines and to calculate their molecular weight. One, two and three S-S bridges per linked fragment mere considered, taking into account the possibility that the fragments contain unabridged residues, The mass data obtained from the spectral analysis of peptide mixtures of the digested protein are then associated to the fitting structures of disulphide-bridged peptides, giving rise to the primary output, This output can then be screened by using information on the specificity of the proteolytic agent(s) used and/or any further mass data provided by Edman degradation and/or carboxypeptidase treatment of the peptide mixtures. The need for such a computer aid is discussed and examples of application are given.

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Documento generato il 20/09/20 alle ore 07:54:02