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Titolo:
SPECIFIC T-CELL LINES FOR OVALBUMIN, OVOMUCOID, LYSOZYME AND 2 OA SYNTHETIC EPITOPES, GENERATED FROM EGG ALLERGIC PATIENTS PBMC
Autore:
HOLEN E; ELSAYED S;
Indirizzi:
HAUKELAND HOSP,DIV BIOCHEM,DEPT BIOL CLIN,ALLERGY RES GRP N-5021 BERGEN NORWAY UNIV HOSP,DIV BIOCHEM,DEPT BIOL CLIN,ALLERGY RES GRP BERGEN NORWAY
Titolo Testata:
Clinical and experimental allergy
fascicolo: 9, volume: 26, anno: 1996,
pagine: 1080 - 1088
SICI:
0954-7894(1996)26:9<1080:STLFOO>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAL-D-I; MAJOR ALLERGENS; IGE ANTIBODIES; POLLEN; CLONES; INTERLEUKIN-4; DETERMINANTS; RECOGNITION; PEPTIDES; RECEPTOR;
Keywords:
ALLERGEN SPECIFIC T CELL LINES; B CELL EPITOPE; CYTOKINE PROFILE; HENS EGG WHITE ALLERGENS; OVALBUMIN 105-122; OVALBUMIN 323-339; OVOMUCOID; T CELL EPITOPES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
E. Holen e S. Elsayed, "SPECIFIC T-CELL LINES FOR OVALBUMIN, OVOMUCOID, LYSOZYME AND 2 OA SYNTHETIC EPITOPES, GENERATED FROM EGG ALLERGIC PATIENTS PBMC", Clinical and experimental allergy, 26(9), 1996, pp. 1080-1088

Abstract

Background Proteins of hen egg white are common ingredients of food and difficult to eliminate. Allergens of egg white induce allergic symptoms among relatively high numbers of patients suffering from food allergy. B cell epitopes to hen egg white major allergens have been reported. Considering that IgE antibody formation is mostly T cell dependent, the study of T cell epitopes is essential for both T cell dependentand independent IgE response. Objectives Little information on T cellepitopes recognizing food allergens has been reported. T cell responses to hen egg white allergens and two synthetic OA peptides located atamino acid residues No. 105-122 and 323-339 were investigated. Methods Peripheral blood mononuclear cells from hen egg allergic patients were investigated. Various allergens of hen egg white were used for stimulation. Primary proliferation responses were detected followed by thegeneration of long-term cultures which were examined for their specificity, phenotype, cytokine profile and IgE production. The allergen specific T cell lines were mapped using a panel of 13 synthetic peptidesof ovalbumin. Results Human T cells recognizing ovomucoid, lysozyme and ovalbumin epitope 105-122 are reported for the first time. The celllines were enriched CD4(+)/CD8(+) T cells (CD2(+) > 95%). Ovomucoid and ovalbumin induced IgE synthesis by a small fraction of B cells (1%)present in the ovalbumin and ovomucoid specific T cell lines. Conclusions Human T cells recognized several egg white allergens and epitopeswithin the ovalbumin molecule. Specific IgE was produced in cultures stimulated with ovalbumin and ovomucoid. OA peptides 105-122 and 323-339 have no affinity to the specific IgE of the two patients; an observation which could be of particular interest regarding the mechanisms of peptide-based immunotherapy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 18:31:12