Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
2 COLD INDUCIBLE GENES ENCODING LIPID TRANSFER PROTEIN LTP4 FROM BARLEY SHOW DIFFERENTIAL RESPONSES TO BACTERIAL PATHOGENS
Autore:
MOLINA A; DIAZ I; VASIL IK; CARBONERO P; GARCIAOLMEDO F;
Indirizzi:
UNIV POLITECN MADRID,ETS INGN AGRON,DEPT BIOTECNOL E-28040 MADRID SPAIN UNIV FLORIDA,LAB PLANT CELL & MOL BIOL GAINESVILLE FL 32611
Titolo Testata:
MGG. Molecular & general genetics
fascicolo: 1-2, volume: 252, anno: 1996,
pagine: 162 - 168
SICI:
0026-8925(1996)252:1-2<162:2CIGEL>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSIENT EXPRESSION ASSAYS; AMMONIA-LYASE GENE; MICROPROJECTILE BOMBARDMENT; NUCLEOTIDE-SEQUENCE; MOLECULAR ANALYSIS; ALEURONE CELLS; PROMOTER; ACID; PROTOPLASTS; ARABIDOPSIS;
Keywords:
BACTERIAL PATHOGENS; BARLEY; LIPID TRANSFER PROTEINS; LOW TEMPERATURE; PROMOTER ANALYSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
A. Molina et al., "2 COLD INDUCIBLE GENES ENCODING LIPID TRANSFER PROTEIN LTP4 FROM BARLEY SHOW DIFFERENTIAL RESPONSES TO BACTERIAL PATHOGENS", MGG. Molecular & general genetics, 252(1-2), 1996, pp. 162-168

Abstract

The barley genes HvLtp4.2 and HvLtp4.3 both encode the lipid transferprotein LTP4 and are less than 1 kb apart in tail-to-tail orientation. They differ in their non-coding regions from each other and from thegene corresponding to a previously reported Ltp4 cDNA (now Ltp4.1). Southern blot analysis indicated the existence of three or more Ltp4 genes per haploid genome and showed considerable polymorphism among barley cultivars. We have investigated the transient expression of genes HvLtp4.2 and HvLtp4.3 following transformation by particle bombardment,using promoter fusions to the beta-glucuronidase reporter sequence. In leaves, activities of the two promoters were of the same order as those of the sucrose synthase (Ss1) and cauliflower mosaic virus 35S promoters used as controls. Their expression patterns were similar, except that Ltp4.2 was more active than Ltp4.3 in endosperm, and Ltp4.3 wasactive in roots, while Ltp4.2 was not. The promoters of both genes were induced by low temperature, both in winter and spring barley cultivars. Northern blot analysis, using the Ltp4-specific probe, indicated that Xanthomonas campestris pv. translucens induced an increase over basal levels of Ltp4 mRNA, while Pseudomonas syringae pv. japonica caused a decrease. The Ltp4.3-Gus promoter fusion also responded in opposite ways to these two compatible bacterial pathogens, whereas the Ltp4.2-Gus construction did not respond to infection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 10:26:48