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Titolo:
THE ROLE OF PHOSPHOINOSITIDE-DERIVED 2ND-MESSENGERS IN OXYTOCIN-STIMULATED PROSTAGLANDIN-F2-ALPHA RELEASE FROM ENDOMETRIUM OF PIGS
Autore:
TYSSELING KA; UZUMCU M; HOAGLAND TA; CRAIN RC; MIRANDO MA;
Indirizzi:
WASHINGTON STATE UNIV,DEPT ANIM SCI PULLMAN WA 99164 WASHINGTON STATE UNIV,DEPT ANIM SCI PULLMAN WA 99164 UNIV CONNECTICUT,DEPT ANIM SCI STORRS CT 06269 UNIV CONNECTICUT,DEPT MOL & CELL BIOL STORRS CT 06269
Titolo Testata:
Domestic animal endocrinology
fascicolo: 5, volume: 13, anno: 1996,
pagine: 411 - 420
SICI:
0739-7240(1996)13:5<411:TROP2I>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
INOSITOL PHOSPHATE TURNOVER; ESTROUS-CYCLE; UTERINE SECRETION; OVINE ENDOMETRIUM; PHOSPHOLIPASE-C; PREGNANCY; PLASMA; HYDROLYSIS; INVITRO; ASSAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
K.A. Tysseling et al., "THE ROLE OF PHOSPHOINOSITIDE-DERIVED 2ND-MESSENGERS IN OXYTOCIN-STIMULATED PROSTAGLANDIN-F2-ALPHA RELEASE FROM ENDOMETRIUM OF PIGS", Domestic animal endocrinology, 13(5), 1996, pp. 411-420

Abstract

The mechanism for prostaglandin (PG) F-2 alpha release from pig endometrium after oxytocin (OT) treatment is unknown. OT may rapidly stimulate inositol (1,4,5)-trisphosphate (IP3) and diacylglycerol (DAG) formation, consistent with the concept of rapid activation of a second-messenger system. In support of this hypothesis, endometrial IP3 levels were increased (P < 0.05) within 0.5 min after treatment with 0.1 mu M OT. In contrast, increased DAG formation was not detected after treatment with OT. However, similar to the stimulation of endometrial PGF(2 alpha) secretion observed after OT treatment (P < 0.001), PGF(2 alpha)release was increased (P < 0.01) after treatment with phorbol-12-myristate-1 3-acetate (PMA), which mimics DAG activation of protein kinaseC. Further, stimulation of endometrial PGF(2 alpha) secretion did notresult from cell death induced by Ph?A or OT because lactate dehydrogenase, a cytosolic marker of cellular integrity, did not leak into themedium after PMA or OT treatment. In contrast. 0.5% saponin (positivecontrol for cell death and concomitant release of lactate dehydrogenase) increased PGF(2 alpha) secretion (P < 0.05) and lactate dehydrogenase release (P < 0.001). These results indicate that OT induces endometrial IP3 production in a rapid manner indicative of a second-messenger system. The finding that increased DAG was not also detected after OT treatment may reflect rapid metabolism or compartmentalized production of DAG involved in the second-messenger stimulation of phospholipase C. The high background of DAG used in the biosynthesis of cellular lipids would obscure the rather small spatially localized changes in DAG levels resulting from the activation of phospholipase C. The findingthat DAG was present at approximately 10 to 20-fold higher levels than IP3 in resting cells was consistent with this conclusion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 06:52:38