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Titolo:
IN-VITRO MODEL OF PARATHYROID HORMONE-RELATED PROTEIN SECRETION FROM MAMMARY CELLS ISOLATED FROM LACTATING COWS
Autore:
OKADA H; SCHANBACHER FL; MCCAULEY LK; WECKMANN MT; CAPEN CC; ROSOL TJ;
Indirizzi:
OHIO STATE UNIV,DEPT VET BIOSCI,COLL VET MED,1925 COFFEY RD COLUMBUS OH 43210 OHIO STATE UNIV,DEPT VET BIOSCI,COLL VET MED COLUMBUS OH 43210 RAKUNO GAKUEN UNIV,SCH VET PATHOL EBETSU HOKKAIDO 069 JAPAN OHIO STATE UNIV,OHIO AGR RES & DEV CTR,DEPT DAIRY SCI WOOSTER OH 00000 UNIV MICHIGAN,DEPT PERIODONT PREVENT & GERIATR ANN ARBOR MI 48109
Titolo Testata:
Domestic animal endocrinology
fascicolo: 5, volume: 13, anno: 1996,
pagine: 399 - 410
SICI:
0739-7240(1996)13:5<399:IMOPHP>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMAL GROWTH-FACTOR; NORMAL HUMAN KERATINOCYTES; LINE IN-VITRO; CALCIUM HOMEOSTASIS; EPITHELIAL-CELLS; PEPTIDE GENE; BOVINE-MILK; BLOOD-FLOW; COLLAGEN GELS; DAIRY-COWS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
H. Okada et al., "IN-VITRO MODEL OF PARATHYROID HORMONE-RELATED PROTEIN SECRETION FROM MAMMARY CELLS ISOLATED FROM LACTATING COWS", Domestic animal endocrinology, 13(5), 1996, pp. 399-410

Abstract

Parathyroid hormone-related protein (PTHrP) is produced by the lactating mammary gland and is present in milk in a biologically active form. The goal of this investigation was to determine if cells cultured from the lactating mammary glands of cows would secrete PTHrP in vitro. Mammary acini were isolated from lactating cows at 1-6 wk after calving, and fresh or cryopreserved mammary acini were cultured for 14 d on Type I collagen. Cultures on thick layers of collagen (2.5 mm) were detached and allowed to contract on Day 6. PTHrP production was measuredby N-terminal radioimmunoassay and bioassay (increased cAMP levels inROS 17/2.8 osteoblast-like cells). The mammary cells reached confluence at Day 6. PTHrP production was low at Day 2 (<0.5 ng/ml) but increased to peak production (2-4 ng/ml) at approximately Day 6 and remainedconstant until Day 14. Immunoreactive and bioactive PTHrP levels in the culture medium correlated well. The cultures produced lactoferrin (2,000-2,300 ng/ml) and alpha(s1)-casein (14-19 ng/ml). Prolactin stimulated PTHrP production approximately 50% on Days 6-14. PTHrP production was increased approximately 100% by treatment with epidermal growth factor (10 ng/ml) for 2 d. Morphologic evaluation of cultures on thick. contracted collagen at Day 14 revealed an inner layer of mammary epithelial cells overlying myoepithelial cells and an outer layer of collagen containing stromal cells. Immunohistochemistry demonstrated positive staining for PTHrP and cytokeratin in both mammary epithelial and myoepithelial cells and alpha-smooth muscle actin in myoepithelial cells. These data demonstrated that cryopreserved mammary tissue from lactating cows could be cultured in vitro and secreted PTHrP in a regulated manner. This in vitro model will be useful to investigate the function and regulation of PTHrP in the lactating mammary gland.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 22:12:01