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Titolo:
UNILINEAGE MODEL OF HEMATOPOIESIS PREDICTS SELF-RENEWAL OF STEM AND PROGENITOR CELLS BASED ON EX-VIVO GROWTH DATA
Autore:
PENG CA; KOLLER MR; PALSSON BO;
Indirizzi:
UNIV CALIF SAN DIEGO,DEPT BIOENGN,9500 GILMAN DR,MAIL CODE 0412 LA JOLLA CA 92093 UNIV MICHIGAN,DEPT CHEM ENGN ANN ARBOR MI 48109 AASTROM BIOSCI INC ANN ARBOR MI 00000
Titolo Testata:
Biotechnology and bioengineering
fascicolo: 1, volume: 52, anno: 1996,
pagine: 24 - 33
SICI:
0006-3592(1996)52:1<24:UMOHPS>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN BONE-MARROW; LIMITING-DILUTION; EXPANSION; CULTURES; INVITRO; MOUSE; PROLIFERATION; MAINTENANCE; GENERATION; CAPACITY;
Keywords:
UNILINEAGE MODEL; TISSUE FUNCTION EX VIVO; HEMATOPOIESIS; STEM CELL EXPANSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
C.A. Peng et al., "UNILINEAGE MODEL OF HEMATOPOIESIS PREDICTS SELF-RENEWAL OF STEM AND PROGENITOR CELLS BASED ON EX-VIVO GROWTH DATA", Biotechnology and bioengineering, 52(1), 1996, pp. 24-33

Abstract

Stem cell models are used to describe the function of several tissues. We present unilineage kinetic description of stem cell models and their application to the analysis of ex vivo hematopoietic cell expansion data. This model has the capability to simulate the total cell number and the number of cells at each stage of differentiation over time as a function of the stem cell self-renewal probability, the growth rate of each subpopulation, and the mature cell death rate. The model predicts experimental observations in perfusion-based hematopoietic bioreactor systems. To obtain net cell expansion ex vivo, the model simulations show that the stem cell self-renewal probability must exceed one-half, thus resulting in net expansion of the stem cell population. Experimental data on long-term culture-initiating cells (LTC-IC) confirm this prediction and the probability of self-renewal is estimated to be0.62 to 0.73. This self-renewal probability, along with the death rate, define a relationship in which the apparent overall growth rate is less than the compartmental growth rate. Finally, the model predicts that cells beyond the stem cell stage of differentiation must self-renew to achieve the level of expansion within the time frame observed in experimental systems. (C) 1996 John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 12:06:04