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Titolo: UNILINEAGE MODEL OF HEMATOPOIESIS PREDICTS SELFRENEWAL OF STEM AND PROGENITOR CELLS BASED ON EXVIVO GROWTH DATA
Autore: PENG CA; KOLLER MR; PALSSON BO;
 Indirizzi:
 UNIV CALIF SAN DIEGO,DEPT BIOENGN,9500 GILMAN DR,MAIL CODE 0412 LA JOLLA CA 92093 UNIV MICHIGAN,DEPT CHEM ENGN ANN ARBOR MI 48109 AASTROM BIOSCI INC ANN ARBOR MI 00000
 Titolo Testata:
 Biotechnology and bioengineering
fascicolo: 1,
volume: 52,
anno: 1996,
pagine: 24  33
 SICI:
 00063592(1996)52:1<24:UMOHPS>2.0.ZU;28
 Fonte:
 ISI
 Lingua:
 ENG
 Soggetto:
 HUMAN BONEMARROW; LIMITINGDILUTION; EXPANSION; CULTURES; INVITRO; MOUSE; PROLIFERATION; MAINTENANCE; GENERATION; CAPACITY;
 Keywords:
 UNILINEAGE MODEL; TISSUE FUNCTION EX VIVO; HEMATOPOIESIS; STEM CELL EXPANSION;
 Tipo documento:
 Article
 Natura:
 Periodico
 Settore Disciplinare:
 Science Citation Index Expanded
 Citazioni:
 44
 Recensione:
 Indirizzi per estratti:



 Citazione:
 C.A. Peng et al., "UNILINEAGE MODEL OF HEMATOPOIESIS PREDICTS SELFRENEWAL OF STEM AND PROGENITOR CELLS BASED ON EXVIVO GROWTH DATA", Biotechnology and bioengineering, 52(1), 1996, pp. 2433
Abstract
Stem cell models are used to describe the function of several tissues. We present unilineage kinetic description of stem cell models and their application to the analysis of ex vivo hematopoietic cell expansion data. This model has the capability to simulate the total cell number and the number of cells at each stage of differentiation over time as a function of the stem cell selfrenewal probability, the growth rate of each subpopulation, and the mature cell death rate. The model predicts experimental observations in perfusionbased hematopoietic bioreactor systems. To obtain net cell expansion ex vivo, the model simulations show that the stem cell selfrenewal probability must exceed onehalf, thus resulting in net expansion of the stem cell population. Experimental data on longterm cultureinitiating cells (LTCIC) confirm this prediction and the probability of selfrenewal is estimated to be0.62 to 0.73. This selfrenewal probability, along with the death rate, define a relationship in which the apparent overall growth rate is less than the compartmental growth rate. Finally, the model predicts that cells beyond the stem cell stage of differentiation must selfrenew to achieve the level of expansion within the time frame observed in experimental systems. (C) 1996 John Wiley & Sons, Inc.
ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 12:06:04