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Titolo:
IDENTIFICATION OF THE MPL GENE ENCODING UDP-N-ACETYLMURAMATE - L-ALANYL-GAMMA-D-GLUTAMYL-MESO-DIAMINOPIMELATE LIGASE IN ESCHERICHIA-COLI AND ITS ROLE IN RECYCLING OF CELL-WALL PEPTIDOGLYCAN
Autore:
MENGINLECREULX D; VANHEIJENOORT J; PARK JT;
Indirizzi:
UNIV PARIS 11,URA 1131 CNRS,BATIMENT 432 F-91405 ORSAY FRANCE TUFTS UNIV,DEPT MOL BIOL & MICROBIOL BOSTON MA 02111
Titolo Testata:
Journal of bacteriology
fascicolo: 18, volume: 178, anno: 1996,
pagine: 5347 - 5352
SICI:
0021-9193(1996)178:18<5347:IOTMGE>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
L-ALANINE AMIDASE; BETA-LACTAMASE INDUCTION; OVER-PRODUCTION; PURIFICATION; PEPTIDES; STRAINS; MUREIN; DELETION; CLONING; AMPD;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
D. Menginlecreulx et al., "IDENTIFICATION OF THE MPL GENE ENCODING UDP-N-ACETYLMURAMATE - L-ALANYL-GAMMA-D-GLUTAMYL-MESO-DIAMINOPIMELATE LIGASE IN ESCHERICHIA-COLI AND ITS ROLE IN RECYCLING OF CELL-WALL PEPTIDOGLYCAN", Journal of bacteriology, 178(18), 1996, pp. 5347-5352

Abstract

A gene, mpl, encoding ate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase was recognized by its amino acid sequence homology with murC as the open reading frame yjfe present at 96 min on the Escherichia coli map, The existence of such an enzymatic activity was predicted from studies indicating that reutilization of the intact tripeptide L-alanyl-gamma-D-glutamyl-meso-diaminopimelate occurred and accounted for well over 30% of new cell wall synthesis. Murein tripeptide ligase activity could be demonstrated in crude extracts, and greatly increased activity was produced when the gene was cloned and expressed under control of the trc promoter, A null mutant totally lacked activity but wasviable, showing that the enzyme is not essential for growth.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 09:43:16