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Titolo:
GAMETOPHYTE-SPECIFIC TRANSPOSITION OF THE MAIZE DS ELEMENT IN TRANSGENIC TOBACCO
Autore:
FIREK S; MARTIN DJ; ROBERTS MR; STURGESS F; SCOTT R; DRAPER J;
Titolo Testata:
Plant journal
fascicolo: 3, volume: 10, anno: 1996,
pagine: 569 - 578
SICI:
0960-7412(1996)10:3<569:GTOTMD>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARABIDOPSIS-THALIANA; INSERTIONAL MUTAGENESIS; ACTIVATOR AC; GERMINAL TRANSPOSITION; DISSOCIATION EXCISION; PLANT TRANSFORMATION; GENE; PROMOTER; FREQUENCY; LOCUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
39
Recensione:
Indirizzi per estratti:
Citazione:
S. Firek et al., "GAMETOPHYTE-SPECIFIC TRANSPOSITION OF THE MAIZE DS ELEMENT IN TRANSGENIC TOBACCO", Plant journal, 10(3), 1996, pp. 569-578

Abstract

To assess the potential advantages of a transposon-tagging system based on gametophyte-specific transposition a fusion between the anther-specific Arabidopsis thaliana apg promoter and the maize Ac transposasegene was constructed and introduced into tobacco. The ability of thistransposase source to activate Ds transposition in a developmentally controlled manner was monitored by crossing to plants harbouring the cell autonomous excision marker gene construct, Ds-SPT. A number of fully green, streptomycin-resistant seedlings resulting from germinal transposition events were observed in the progeny of apg-TPase x Ds-SPT F1 plants. Streptomycin-resistant sectors were not observed in either F-1 seedlings or F-2 progeny, indicating a complete lack of somatic excision. Further crosses of apg-TPase sources to plants containing Ds-bar herbicide selection excision marker constructs gave reproducible gametophytic excision frequencies of up to 0.3%. Sequencing of Ds excision sites from F-2 seedlings derived from single F-1 plants revealed various sequence alterations in the original Ds insertion 'footprint' indicative of independent Ds excision events. Independent re-insertion was confirmed by Southern analysis of F-2 siblings. It is concluded thatapg-controlled Ac transposase expression activates male gametophyte-specific Ds transposition.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 16:14:05