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Titolo:
CELLULAR-LOCALIZATION AND REGULATION OF CHIF IN KIDNEY AND COLON
Autore:
CAPURRO C; COUTRY N; BONVALET JP; ESCOUBET B; GARTY H; FARMAN N;
Indirizzi:
UNIV PARIS 07,INSERM U246,INST FEDERAT RECH CELLULES EPITHELIALES,BP 146 F-75870 PARIS 18 FRANCE UNIV PARIS 07,INSERM U246,INST FEDERAT RECH CELLULES EPITHELIALES F-75870 PARIS 18 FRANCE UNIV PARIS 07,INSERM U426,INST FEDERAT RECH CELLULES EPITHELIALES F-75870 PARIS 18 FRANCE WEIZMANN INST SCI,DEPT MEMBRANE RES & BIOPHYS IL-76100 REHOVOT ISRAEL
Titolo Testata:
American journal of physiology. Cell physiology
fascicolo: 3, volume: 40, anno: 1996,
pagine: 753 - 762
SICI:
0363-6143(1996)40:3<753:CAROCI>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDULLARY COLLECTING DUCT; RAT DISTAL COLON; H-K-ATPASE; XENOPUS OOCYTES; CLONING; EXPRESSION; PROTEIN; ALDOSTERONE; ACID; NA+;
Keywords:
COLLECTING DUCT; CORTICOSTEROID HORMONES; POTASSIUM DEPLETION; ACIDOSIS; HYDROGEN-POTASSIUM-ADENOSINE-TRIPHOSPHATASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
C. Capurro et al., "CELLULAR-LOCALIZATION AND REGULATION OF CHIF IN KIDNEY AND COLON", American journal of physiology. Cell physiology, 40(3), 1996, pp. 753-762

Abstract

Channel inducing factor (CHIF) is a novel cDNA recently cloned from arat distal colon cDNA library of dexamethasone-treated animals. Whileits expression in Xenopus oocytes evokes a potassium channel activitysimilar to that induced by Isk. (minK), its cellular role is not clear. CHIF exhibits significant homologies with proteins that are putatively regulatory (phospholemman, gamma-subunit of Na+-K+-ATPase, Mat-8) while it differs from the small-conductance potassium channel Isk. We have studied the tissue specificity of CHIF expression in rat by in situ hybridization. CHIF is selectively present in the distal parts of the nephron (medullary and papillary collecting ducts and end portions of cortical collecting tubule) and in the epithelial cells of the distal colon. No expression of CHIF was found in renal proximal tubule, loop of Henle and distal tubule, proximal colon, small intestine, lung, choroid plexus, salivary glands, or brain. To gain some insight into CHIF function, we have investigated, using in situ hybridization and ribonuclease protection assay, whether CHIF mRNA expression could be altered in some situations. In the distal colon, corticosteroid hormones,sodium restriction, low-potassium diet, and metabolic acidosis significantly increased CHIF mRNA expression. In the kidney, metabolic acidosis was the only condition that showed an increase in CHIF mRNA expression. Some of these treatments also altered the expression of the colonic H+-K+-ATPase mRNA. In summary, CHIF mRNA is selectively expressed in the medullary collecting duct of the kidney and in the epithelium of the distal colon; its expression varies differently in these two target tissues after alterations in corticosteroid status, potassium depletion, and metabolic acidosis. The precise cell-specific functions of CHIF remain to be established.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 22:13:35